显微介导中国对虾基因鲤转录组测序及差异表达基因分析  

Transcriptome Sequencing and Differentially Expressed Gene Analysis of Common Carp(Cyprinus carpio)With Microinjection of Chinese Shrimp(Fenneropenaeus chinensis)DNA

作　　者：闫学春[1] 栾培贤[1] 何立川 YAN Xuechun;LUAN Peixian;HE Lichuan(National Local Joint Engineering Laboratory for Freshwater Fish Breeding,Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture and Rural Affairs,Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)

机构地区：[1]中国水产科学研究院黑龙江水产研究所,淡水鱼类育种国家地方联合工程实验室,农业农村部淡水水产生物技术与遗传育种重点实验室,黑龙江哈尔滨150070

出　　处：《水产学杂志》2022年第5期1-7,共7页Chinese Journal of Fisheries

基　　金：中国水产科学研究院基本科研业务费资助(2020TD22);中央级公益性科研院所基本科研业务费专项(HSY201707M);国家高技术研究发展计划(863计划)项目(2011AA100404).

摘　　要：运用显微介导的远缘杂交技术,将中国明对虾(Fenneropenaeus chinensis)总DNA直接导人鲤(Cyprinus carpio)受精卵内,创建鲤外源DNA导入系。利用Illumina高通量测序平台,对未进行显微介导和显微介导中国明对虾基因的2种鲤的肌肉组织进行转录组测序分析,结果获得差异表达基因417个,并对其进行GO(Gene Ontology)功能注释和KEGG代谢通路注释,得到18个与氨基酸相关的差异表达基因,其中14个下调基因,4个上调基因。分析发现,其中有2个基因在氨基酸代谢通路中能引起氨基酸含量的升高,即氨甲酰-磷酸合成酶(CPS2)基因和脯氨酰4-羟化酶(P4H)基因。对这2个基因进行实时荧光定量PCR验证发现,其基因的表达趋势与转录组结果一致,从而证实了转录组的分析结果。The total DNA of Chinese shrimp Fenneropenaeus chinensis as an exogenous donor was directly introduced into the fertilized eggs of the carp Cyprinus carpio using a microscopically mediated distant hybridization technique.The transcriptome of transgenic carp muscle tissue was then sequenced by the Illumina sequencing method,the muscle tissue of the common carp without transgenic operation as control group.A total of 417 differentially expressed genes(DEGs)were found and GO(gene ontology)functional annotation and KEGG metabolic pathway annotation were performed for these DEGs,in which 18 DEGs were proved to be related to amino acids,14 down regulated genes and 4 up-regulated genes.Real time PCR revealed that gene expression patterns of these two genes,carbamyl phosphate synthase(CPS2)and prolyl 4-hydroxylase(P4H),were consistent with the transcriptome analysis results,which confirmed the conclusion of transcriptome analysis.

关 键 词：显微介导 中国对虾总DNA 转录组测序 差异表达基因 

分 类 号：S917.4[农业科学—水产科学] Q959[生物学—动物学]