产气荚膜梭菌分子分型研究进展  

Progress in research of molecular typing of Clostridium perfringens

作　　者：钟佳鑫 王媛媛 白璐璐 郑浩然 周海健[1] 吴媛[1] 卢金星[1] Zhong Jiaxin;Wang Yuanyuan;Bai Lulu;Zheng Haoran;Zhou Haijian;Wu Yuan;Lu Jinxing(State Key Laboratory of Communicable Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Shaanxi University of Traditional Chinese Medicine,Xi'an 710000,Shaanxi,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206 [2]陕西中医药大学,陕西西安710000

出　　处：《疾病监测》2022年第9期1248-1256,共9页Disease Surveillance

摘　　要：产气荚膜梭菌是自然界常见的致病菌,广泛分布于人类和动物的胃肠道、食品和环境中,已被美国、欧盟部分国家及日本列为食源性疾病暴发的主要原因之一。近年来产气荚膜梭菌感染在全球呈上升趋势。目前该菌的分子流行病学方面研究较多,常用的分子分型方法包括脉冲场凝胶电泳、多位点序列分型、核心基因多位点序列分型、单核苷酸多态性分析、毒素分型等。分子分型技术对研究产气荚膜梭菌的暴发溯源、流行情况、遗传演化规律与种群特征具有重要意义,同时分子分型方法的不断创新与改进能够提高产气荚膜梭菌感染检测与诊断的有效性、准确性。Clostridium perfringens is a common pathogenic bacterium found in the human gastrointestinal tract,animals,food,and the environment.It has been identified as one of the major causes of foodborne disease outbreaks in the United States,some EU countries and Japan.In recent years,the frequency of C.perfringens infection has been increasing remarkably around the world.At present,there are many studies of molecular epidemiology of C.perfringens.The molecular typing methods commonly used include pulse field gel electrophoresis,multi-site sequence analysis,core gene multi-site sequence typing,toxin typing,and whole genome sequencing analysis.As the development of molecular typing methods,the effectiveness and accuracy improvements of the diagnosis and investigation of C.perfringens infection become possible,which can provide data basis for studying the transmission mechanism,epidemiological investigation,evolution and species characteristics of C.perfringens.

关 键 词：产气荚膜梭菌 分子分型 多位点序列分型 脉冲场凝胶电泳 分子流行病学 

分 类 号：R211[医药卫生—中医学] R372