参附注射液干预对热射病大鼠肠道免疫凝血及肝肾功能的影响  

Effects of Shenfu injection intervention on intestinal immune,blood coagulation,liver and kidney functions in rats with heat stroke

作　　者：黄翠兰[1] 黄定兴 杨春丽[1] Huang Cuilan;Huang Dingxing;Yang Chunli(Department of Critical Care Medicine,Jiangxi Provincial People's Hospital,Nanchang 330006,Jiangxi,China;School of Medicine,Xi'an Jiaotong University,Xi'an 710000,Shaanxi,China)

机构地区：[1]江西省人民医院重症医学科,南昌330006 [2]西安交通大学医学院,陕西西安710000

出　　处：《中国中西医结合急救杂志》2022年第4期435-439,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：江西省中医药科研课题(2019A196)。

摘　　要：目的探讨参附注射液(SFI)对热射病(HS)大鼠凝血功能、肝肾功能及肠道免疫功能的影响。方法选择90只SPF级雄性SD大鼠,用随机数字表法分为常温对照组、HS模型组和SFI干预组,每组30只。将制模大鼠置于仿真气候舱内,舱内温度(40.0±0.5)℃、湿度(60±5)%,持续热暴露,直肠温度达42.0℃以上取出置于室温;常温对照组大鼠置于室温(25.0±2.0)℃、湿度(50±5)%的环境中,不给予任何处理。制模大鼠去除热应激后经股静脉给予4℃林格液进行液体复苏;模型组同时注射生理盐水4 mL/kg,SFI干预组则注射SFI 5 mL/kg。于实验后1、3、7 d取大鼠下腔静脉血,处死大鼠取肠黏液和肠黏膜组织;采用生化分析仪检测大鼠凝血功能和肝肾功能,采用酶联免疫吸附试验(ELISA)法测定肠黏液中分泌型免疫球蛋白A(sIgA)水平,用流式细胞仪检测回肠黏膜组织中CD4^(+)、CD8^(+)T淋巴细胞百分比,计算CD4^(+)/CD8^(+)比值。结果与常温对照组比较,HS模型组大鼠凝血酶原时间(PT)、部分活化凝血酶原时间(APTT)、凝血酶时间(TT)均明显延长,纤维蛋白原(FIB)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、血尿素氮(BUN)、血尿酸(UA)和血肌酐(SCr)呈逐渐升高趋势,而总蛋白(TP)、白蛋白(ALB)、sIgA、CD4^(+)/CD8^(+)比值逐渐降低。SFI干预组治疗7 d后上述指标明显改善,与HS模型组比较,SFI干预组PT、APTT、TT时间明显缩短〔PT(s):15.14±0.15比18.07±0.42,APTT(s):32.25±0.90比44.41±0.70,TT(s):37.11±0.07比45.88±0.78,均P<0.05〕,FIB、ALT、AST、ALP、BUN、UA、SCr水平均明显降低〔FIB(g/L):6.73±0.41比8.78±0.26,ALT(U/L):121.21±0.39比253.78±1.62,AST(U/L):205.07±0.39比342.95±0.74,ALP(U/L):327.06±0.32比463.74±1.44,BUN(μmol/L):45.30±0.58比55.28±1.47,UA(μmol/L):146.23±2.18比165.61±0.80,SCr(μmol/L):18.47±0.61比21.74±1.04,均P<0.05〕,TP、sIgA、CD4^(+)/CD8^(+)比值明显升高〔TP(g/L):81.41±0.78比74.10±0.Objective To explore the effect of Shenfu injection(SFI)on blood coagulation,liver,kidney and intestinal immune functions in rats with heat stroke(HS).Methods Ninety specific pathogen free(SPF)male Sprague-Dawley(SD)rats were randomly divided into 3 groups:normal temperature control group,HS model group and SFI intervention group,with 30 rats in each group.The model rats were placed in a simulated climate cabin with temperature of(40.0±0.5)℃and humidity of(60±5)%,and persistently exposed to heat until the rectal temperature above 42.0℃,then taken out and placed at room temperature.The rats in normal temperature control group were placed at room temperature(25.0±2.0)℃and humidity(50±5)%without any treatment.The HS rats were given ringer's solution at 4℃through femoral vein for fluid resuscitation after heat stress was removed,then 4 mL/kg normal saline was injected into the rates of HS model group,5 mL/kg SFI was injected into the rats of SFI intervention group.On the 1st,3rd and 7th day after the experiment,the blood of the inferior vena cava of rats was taken,and the rats were sacrificed to take intestinal mucous membrane tissue and its mucous fluid;biochemical analyzer was used to detect the indexes related to blood coagulation function and liver and kidney functions in rats,enzyme linked immunosorbent assay(ELISA)was used to determine secretory immunoglobulin A(sIgA)level in ileum mucus supernatant,flow cytometry was used to detect CD4^(+),CD8^(+)T lymphocytes in ileum mucous membrane tissue,and CD4^(+)/CD8^(+)ratio was calculated.Results Compared with the normal temperature control group,prothrombin time(PT),activated partial thromboplastin time(APTT)and thrombin time(TT)in HS model group were significantly prolonged,the fibrinogen(FIB),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),blood urea nitrogen(BUN),blood uric acid(UA)and blood serum creatinine(SCr)presented a tendency of gradually increasing;while the total protein(TP),albumin(ALB),sIgA and ratio o

关 键 词：热射病 参附注射液 凝血功能 肝功能 肾功能 肠道 免疫功能 

分 类 号：R285[医药卫生—中药学]