PMCA2基因多态性与噪声性听力损失易感性研究  

Study on the relationship between PMCA2 polymorphism and susceptibility to noise-induced hearing loss

作　　者：谢春姣[1] 李燕茹[1] 黄丽丽[1] 李艳华[1] 刘影玫[1] 刘移民[1,2] XIE Chun-jiao;LI Yan-ru;HUANG Li-li;LI Yan-hua;LIU Ying-mei;LIU Yi-min(Guangzhou Occupational Disease Prevention and Treatment Hospital,Guangzhou,Guangdong 510620,China;不详)

机构地区：[1]广州市职业病防治院,广东广州510620 [2]广州医科大学职业与环境卫生研究所,广东广州510180

出　　处：《中国职业医学》2022年第3期260-264,共5页China Occupational Medicine

基　　金：广东省医学科学技术研究基金项目(A2020340);广州市医学重点学科建设项目(2021—2023年);广州市高水平临床重点专科建设项目(穗卫函[2019]1555号);广州市“121人才梯队工程”后备人才项目(穗人社发[2011]167号);广州市卫生健康科技项目(20211A011047)。

摘　　要：目的探讨质膜钙离子-三磷酸腺苷酶2异构体(PMCA2)基因多态性与噪声性听力损失(NIHL)易感性的关系。方法采用单纯随机抽样方法,选择228名NIHL工人为病例组,以230名年龄、噪声作业工龄、噪声接触水平等均相近的听力正常工人为对照组。采集2组人群周围血提取DNA,采用MassArray系统进行PMCA2单核苷酸多态性(SNP)位点基因分型。结果PMCA2基因rs1719571和rs14154位点在对照组人群等位基因频率分布均符合遗传学Hardy-Weinberg平衡定律(P值均>0.05)。2组人群rs1719571、rs14154位点不同基因型分布和等位基因频率分布分别比较,差异均无统计学意义(P值均>0.05)。在排除年龄、噪声接触工龄、个体噪声接触强度、吸烟和饮酒的影响后,携带rs1719571位点GA基因型的个体罹患NIHL的风险低于携带GG基因型者(比值比=0.53,95%置信区间=0.31~0.90,P<0.05);rs14154位点多态性与NIHL发病风险无关联(P>0.05)。结论PMCA2 rs1719571位点SNP与NIHL易感性相关;GA基因型可能是NIHL发生的潜在保护因素。objectiveTo investigate the correlation between genetic polymorphism of plasma membrane Ca2+-ATPase isoform 2(PMCA2)and susceptibility to noise-induced hearing loss(NIHL).MethodsA total of 228 workers with NIHL were selected asthe case group by simple random sampling method,and 230 normal hearing workers with similar age,length and level of noiseexposure were selected as the control group.DNA was extracted from peripheral blood of workers of the two groups,and singlenucleotide polymorphism(SNP)of PMCA2 was genotyped by Mass Array system.ResultsThe allele frequencies of rs1719571and rs14154 of PMCA2 gene in the control population were consistent with Hardy-Weinberg equilibrium(both P>0.05).Therewas no significant difference in the distribution of rs1719571 and rs14154 genotypes and allele frequencies between the twogroups(all P>0.05).Logistic regression analysis showed that the risk of NIHL in GA genotype of rs1719571 was lower than thatin GG genotype(odds ratio=0.53,95%confidence interval=0.31-0.90,P<0.05),after excluding the effects of age,length ofexposure to noise,intensity of exposure to noise,smoking and alcohol consumption.The genotype of SNP rs14154 might notcontribute to the genetic susceptibility of NIHL(P>0.05).ConclusionThe SNP of PMCA2 rs1719571 is associated with thesusceptibility of NIHL,and GA genotype may be a potential protective factor for NIHL.

关 键 词：噪声 听力损失 基因多态性 PMCA2基因 易感性 保护因素 

分 类 号：R135.8[医药卫生—劳动卫生]