白芍多糖对化学性肝损伤肝阴虚证病证结合模型的影响和作用机制  

Performance and Mechanism of Paeoniae Radix Alba Polysaccharides in Treatment of Chemical-induced Liver Injury with Syndrome of Liver Yin Deficiency

作　　者：贾岚 高晶[1] 孟智睿 魏益谦 李丽[1] 邵紫萱 高学敏[1] 王景霞[1] JIA Lan;GAO Jing;MENG Zhirui;WEI Yiqian;LI Li;SHAO Zixuan;GAO Xuemin;WANG Jingxia(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界中医药》2022年第21期3019-3025,共7页World Chinese Medicine

基　　金：国家自然科学基金项目(82074036)——基于调节“环核苷酸-内分泌-细胞因子网络系统”研究白芍养肝阴的物质基础与作用机制;国家中医药管理局中医药行业科研专项(201507004)——含毒性及疑似毒性药材中成药的有效性和安全性文献研究。

摘　　要：目的:在建立肝阴虚证病证结合实验模型的基础上,研究白芍多糖对大鼠化学性肝损伤肝阴虚证候模型的影响和作用机制。方法:将40只无特定病原体(Specific Pathogen Free,SPF)级雄性SD大鼠,按体质量随机区组法分为空白组、模型组、阳性药组、白芍多糖组。除空白组外,其余各组大鼠腹腔注射浓度20%CCl 4橄榄油溶液,后期给予附子、干姜、肉桂温热中药复方灌胃,同时给药,连续6周。6周后观察大鼠体质量,易激惹程度,毛发光泽度,小便颜色,大便颜色质地,肛温。测定血清及肝组织转氨酶活性、细胞因子水平、脂肪代谢相关酶活性、抗氧化酶活性并取肝脏进行HE病理切片检查。结果:与模型组比较,多糖组可改善大鼠大便的干燥程度(P<0.05),血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、Ⅳ型胶原(CⅣ)、层粘连蛋白(LN)水平显著降低(P<0.05),丙二醛水平显著下降(P<0.05),谷胱甘肽过氧化物酶(GSH-Px)活力显著升高(P<0.05),病理切片有所改善。白芍多糖组大鼠TNF-α、IL-6水平显著下降(P<0.05),磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶B(AKT)mRNA表达量显著下降(P<0.05)。结论:白芍提取物白芍多糖具有保护化学性肝损伤肝阴虚证的功效,其作用机制可能与抗肝纤维化、减轻氧化应激水平、调节细胞因子平衡有关。Objective:To study the performance and mechanism of Paeoniae Radix Alba polysaccharides(PRAP)in treating the rat model of chemical-induced liver injury with the syndrome of liver yin deficiency.Methods:Forty SPF-grade male Sprague-Dawley rats weighing(200±20)g were randomly assigned into a blank control group,a model group,a positive drug group,and a PRAP group.The rats in the other groups except the blank control group were intraperitoneally injected with 20% CCl_(4) olive oil solution,and later treated with a Chinese herbal compound prescription composed of Aconiti Lateralis Radix Praeparata,Zingiberis Rhizoma,and Cinnamomi Cortex for 6 weeks.After 6 weeks,the rats were observed for irritability,hair gloss,urine color,and stool color and texture,and the body weight and rectal temperature were measured.The levels of glutamic-pyruvic transaminase(GPT),glutamic-oxalacetic transaminase(GOT),type IV collagen(CIV),laminin(LN),tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),as well as the activity of glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA)were measured.Hematoxylin-eosin(HE)staining was performed for the pathological examination of the liver tissue.The mRNA levels of phosphatidylinositol-3-kinase(PI3K)and protein kinase B(AKT)were determined.Results:Compared with the model group,PRAP moistened the stool dryness(P<0.05),lowered the serum levels of GPT,GOT,CIV,and LN(P<0.05),decreased the content of MDA(P<0.05),increased the activity of GSH-Px(P<0.05),and alleviated the pathological damage.Moreover,it lowered the levels of TNF-αand IL-6(P<0.05)and down-regulated the mRNA levels of PI3K and AKT(P<0.05).Conclusion:PRAP may protect rat from the chemical-induced liver injury with the syndrome of liver yin deficiency by alleviating fibrosis,mitigating oxidative stress,and regulating cytokine levels.

关 键 词：白芍多糖 化学性肝损伤 肝阴虚证 病证结合 肝纤维化 氧化应激 磷脂酰肌醇-3-激酶/蛋白激酶B 细胞因子 

分 类 号：R284[医药卫生—中药学] R575[医药卫生—中医学]