氯雷他定与消风止痒颗粒对3种荨麻疹动物模型的药效评价研究  

Efficacy of Loratadine and Xiaofeng Zhiyang Granules on Three Animal Models of Urticaria

作　　者：庞博[1] 徐英莉 刘晓峰 陈梦苹 王雅欣 姚荣妹 周利润 曹姗 崔晓兰[1] PANG Bo;XU Yingli;LIU Xiaofeng;CHEN Mengping;WANG Yaxin;YAO Rongmei;ZHOU Lirun;CAO Shan;CUI Xiaolan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Liaoning Vocational College of Medicine,Liaoning 110101,China;Chinese Medical College of Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]中国中医科学院中药研究所生物安全实验室,北京100700 [2]辽宁医药职业学院,沈阳110101 [3]天津中医药大学中医药学院,天津301617

出　　处：《世界中医药》2022年第21期3026-3032,共7页World Chinese Medicine

基　　金：国家重点研发计划项目(2021YFC1712903);中国中医科学院科技创新工程项目(CI2021A04606)。

摘　　要：目的:探究氯雷他定和消风止痒颗粒对免疫性/非免疫性接触/非接触性慢性荨麻疹模型的药效作用。方法:采用尾静脉注射抗二硝基苯基(DNP)-IgE单克隆抗体进行致敏,双耳涂抹2,4-二硝基氟苯(DNFB)进行激发造成小鼠免疫性接触性慢性荨麻疹模型;腹腔注射卵白蛋白-氢氧化铝混悬液进行致敏及激发诱导致大鼠免疫性非接触性慢性荨麻疹模型。二甲基亚砜涂抹豚鼠耳郭两面致非免疫性接触性荨麻疹模型。结果:免疫性接触性/非接触性慢性荨麻疹模型试验中,氯雷他定和消风止痒颗粒均能显著减少小鼠的抓挠次数,抑制大、小鼠腹腔肥大细胞脱颗粒(均P<0.05);氯雷他定能明显缓解炎症介质的释放,消风止痒颗粒则能显著抑制背部风团的发生,减轻耳肿胀(均P<0.05)。非免疫性接触性荨麻疹模型实验中,消风止痒颗粒可显著减轻豚鼠耳肿胀度及耳厚度(均P<0.05),缓解变态反应肿胀症状。结论:氯雷他定对免疫性慢性荨麻疹有良好的治疗效果,可抑制炎症介质的释放,缓解瘙痒症状。消风止痒颗粒则对接触性荨麻疹作用明显,有缓解皮肤红肿,抑制风团之效。Objective:To investigate the efficacy of loratadine and Xiaofeng Zhiyang Granules on immunologic/non-immunologic contact/non-contact chronic urticaria models.Methods:The immunologic contact chronic urticaria mouse model was induced by tail vein injection of anti-dinitrophenyl(DNP)-IgE monoclonal antibody for sensitization and excitation with 2,4-dinitrofluorobenzene(DNFB)applied to both ears.The immunologic non-contact chronic urticaria rat model was induced by intraperitoneal injection of ovalbumin-aluminium hydroxide suspension for sensitization and excitation.A model of non-immunologic contact urticaria in guinea pigs was induced by application of dimethyl sulfoxide to both sides of the ears.Results:In the immunologic contact/non-contact chronic urticaria models,loratadine and Xiaofeng Zhiyang Granules reduced the number of scratching and inhibited mast cell degranulation in the abdominal cavity of mice and rats(P<0.05);loratadine alleviated the release of inflammatory mediators,while Xiaofeng Zhiyang Granules inhibited the occurrence of wheal on the back and reduced ear swelling(P<0.05).In the non-immunologic contact urticaria model,Xiaofeng Zhiyang Granules reduced ear swelling and ear thickness in guinea pigs(P<0.05)and mitigated the symptoms of allergic swelling.Conclusion:Loratadine was effective in the treatment of immunologic chronic urticaria,which inhibited the release of inflammatory mediators and relieved itching symptoms.Xiaofeng Zhiyang Granules had marked effect on contact urticaria,which relieved skin redness and swelling and suppressed wheal.

关 键 词：免疫性荨麻疹 接触性荨麻疹 肥大细胞 炎症介质 氯雷他定 消风止痒颗粒 实验研究 

分 类 号：R275.9[医药卫生—中西医结合]