黑老虎不同部位酚酸类成分差异分析  

Differential Analysis of Phenolic Acids from Different Parts of Kadsura coccinea

作　　者：高渐飞[1] 周玮[2] 刘妮 GAO Jian-fei;ZHOU Wei;LIU Ni(Guizhou Mountainous Resources Institute in Guizhou Academy of Sciences,Guiyang 550001,China;Guizhou Industry Polytechnic College,Guiyang 550008,China)

机构地区：[1]贵州科学院山地资源研究所,贵州贵阳550001 [2]贵州工业职业技术学院,贵州贵阳550008

出　　处：《中药材》2022年第2期351-358,共8页Journal of Chinese Medicinal Materials

基　　金：贵州省科技计划重大专项(黔科合平台人才[2017]5411号);国家重点研发计划项目(2016YFC0502601)。

摘　　要：目的:研究黑老虎酚酸类化学成分及其在根、茎、叶中的分布和富集差异。方法:利用UPLC和MS/MS、Q TRAP采集质谱信息,基于自建数据库MVDB V2.0和代谢物信息公共数据库、MRM获取代谢物数据;利用软件Analyst 1.6.3对质谱数据进行归一化处理和注释,同时对大部分物质使用对照品进一步确认,基于鉴定出的代谢物结构配置进行分类。结果:从黑老虎植株中鉴定出137个酚酸代谢物,其中68个获得KEGG标注;叶和茎都分别含有125个、根中103个,3个部位共有94个。3组对比(叶vs茎、叶vs根、茎vs根)差异代谢物总数为126个,在均为差异代谢物(44个)中有19个呈连续下调模式,仅有3个向上表达。结论:黑老虎叶和茎中的酚酸类代谢物数量相同、积累量接近,而根中数量大幅减少,表达量明显下调;绿原酸、咖啡酸、阿魏酸、没食子酸等具有重要药理活性的化合物在黑老虎植株中检出。Objective: To study the chemical constituents of phenolic acids and their distribution and enrichment in roots, stems and leaves of Kadsura coccinea.Methods: The UPLC,MS/MS and Q TRAP methods were used to collect the mass spectrum information, and the metabolite data were obtained based on the self-built database MVDB V2.0,public metabolite information database and MRM.The Analyst 1.6.3 software was used to normalize and annotate the mass spectrum data.At the same time, the most substances were further confirmed with reference materials and classified based on the identified metabolite structure configuration.Results: 137 phenolic acid metabolites were identified from Kadsura coccinea plants, among which 68 were KEGG labeled.There were 125,125,103 metabolites in leaves, stems, roots, respectively and 94 metabolites in three parts.There were 126 differential metabolites in the three groups(leaves and stems, leaves and roots, stems and roots),19 of the 44 differential metabolites showed a continuous down-regulation pattern, and only 3 were up-regulated.Conclusion: The number of phenolic acid metabolites in leaves and stems of Kadsura coccinea is the same, and the accumulation amount is similar, but the number of phenolic acid metabolites in roots is significantly reduced, and the expression is obviously down-regulated.The chlorogenic acid, caffeic acid, ferulic acid, gallic acid and other compounds with important pharmacological activities are detected in Kadsura coccinea.

关 键 词：代谢组学 酚酸 黑老虎 不同部位 分布规律 

分 类 号：R284.2[医药卫生—中药学]