口蹄疫病毒抗体SPC-ELISA定性检测与LPB-ELISA定量检测方法的比较  

Comparison of SPC-ELISA Qualitative Detection and LPB-ELISA Quantitative Detection for Antibodies against FMDV

作　　者：蔡文博 Cai Wenbo(Jintan District Center for Animal Disease Prevention and Control,Changzhou,Jiangsu 213200,China)

机构地区：[1]常州市金坛区动物疫病预防控制中心,江苏常州213200

出　　处：《中国动物检疫》2022年第12期118-122,共5页China Animal Health Inspection

摘　　要：为评估固相竞争ELISA(SPC-ELISA)定性检测与液相阻断ELISA(LPB-ELISA)定量检测口蹄疫病毒(foot and mouth disease virus,FMDV)抗体的符合度和一致性,以2022年春季从规模猪场、牛场、羊场采集的300份血液样本为试验对象,分别应用SPC-ELISA定性和LPB-ELISA定量检测方法进行O、A型FMDV抗体检测,对检测结果应用统计软件GraphPad Prism 8中的χ~2检验进行差异显著性统计与分析,采用Kappa统计量分析两种ELISA检测方法的一致性。结果显示:SPC-ELISA检测两种抗体的阳性率总体均略高于LPBELISA,但差异不显著(P> 0.05)。两种方法检测O型FMDV抗体总符合率为94.81%,Kappa值为0.8;检测A型FMDV抗体总符合率为91.48%,Kappa值为0.7。结果表明,两种方法检测结果符合度高,且高度一致。因此,在FMDV抗体检测时可根据实际需要任意选择其中一种使用。本研究为技术人员在实际工作中科学选择检测方法提供了参考。In order to evaluate the conformity and consistency of solid-phase-competitive ELISA(SPC-ELISA)qualitative detection and liquid-phase-blocking ELISA(LPB-ELISA)quantitative detection for antibodies against foot-and-mouth disease virus(FMDV),a total of 300 blood samples collected from large-scale pig,cattle and sheep farms in spring 2022 were detected for antibodies against FMDV types O and A,followed by difference significance statistics and analysis on the results by χ~2 test in GraphPad Prism 8,and by analysis on the consistency of the two ELISA methods by Kappa statistic. The results showed that the positive rates of the two kinds of antibodies detected by SPC-ELISA were slightly higher than those by LPB-ELISA,the difference was not significant(P > 0.05). The total coincidence rate of detection of antibodies against FMDV type O by the two methods was 94.81%,and the Kappa value was 0.8;while that of antibodies against FMDV type A was 91.48%,and the Kappa value was 0.7. In conclusion,the results obtained by the two methods were highly consistent with each other,it was therefore recommended to select an appropriate method according to actual conditions,and a reference was provided for technicians in their practice.

关 键 词：口蹄疫病毒 抗体检测 液相阻断ELISA 固相竞争ELISA 

分 类 号：S851.34[农业科学—预防兽医学]