猪流行性腹泻病毒N蛋白单克隆抗体的制备与鉴定  

Preparation and Identification of Monoclonal Antibody against N Protein of Porcine Epidemic Diarrhea Virus

作　　者：韩笑 庞俊增 李林岳 王亚楠[1] 范国英[1] 李任峰[1] 王自良[1] HAN Xiao;PANG Jun-zeng;LI Lin-yue;WANG Ya-nan;FAN Guo-ying;LI Ren-feng;WANG Zi-liang(Henan Institute of Science and Technology,College of Animal Science and Veterinary Medicine,Xinxiang,Henan,453003,China)

机构地区：[1]河南科技学院动物科技学院,河南新乡453003

出　　处：《动物医学进展》2022年第12期10-14,共5页Progress In Veterinary Medicine

基　　金：河南省科技攻关项目(202102110255,212102110098)。

摘　　要：为制备猪流行性腹泻病毒(PEDV)单克隆抗体,采用生物信息学方法对CH/HNQX-3/14毒株N蛋白的抗原表位进行分析,将合成表位肽与载体偶联后免疫Balb/c小鼠,通过杂交瘤技术获得1株能稳定分泌抗PEDV单克隆抗体的杂交瘤细胞株(命名为9C4),其分泌抗体属于IgG2b亚类,轻链为κ链,腹水效价为1∶1.28×10^(4),能特异性识别PEDV。该单克隆抗体为研究PEDV N蛋白的功能和建立诊断方法提供了重要材料。To prepare monoclonal antibody against PEDV,in this study,the antigenic epitopes of N protein from PEDV strainCH/HNQX-3/14 were analyzed using bioinformatics tools.Then the synthetic epitope peptides were coupled with keyhole limpethemocyanin(KLH)and used to immunize Balb/c mice.The monoclonal antibody(designated as mAb 9C4)against PEDV was obtained by hybridoma technique.The mAb belongs to IgG2b subclass and the light chain isκtype.The titer of ascites for 9C4 was 1∶1.28×10^(4) and could specifically recognize PEDV.This mAb would provide valuable tools for the study of PEDV N protein function and the establishment of PEDV diagnostic methods in the future.

关 键 词：猪流行性腹泻病毒 核衣壳蛋白 合成表位 单克隆抗体 

分 类 号：S852.659.6[农业科学—基础兽医学] S858.28[农业科学—兽医学]