达氟沙星多克隆抗体制备及间接竞争ELISA方法的建立  

Preparation of Polyclonal Antibodies against Darfloxacin and Establishment of ic-ELISA Method

作　　者：张欣欣 齐永华[1] 岳锋 路少鹏[1] 李文明 郭东光 潘鹏涛[1] 王选年[1] ZHANG Xin-xin;QI Yong-hua;YUE Feng;LU Shao-peng;LI Wen-ming;GUO Dong-guang;PAN Peng-tao;WANG Xuan-nian(Henan Engineering Laboratory for Molecular Diagnosis of Animal Diseases,Xinxiang University,Xinxiang,Henan,453000,China;Zhengzhou University,Zhengzhou,Henan,450000,China)

机构地区：[1]新乡学院动物疫病分子诊断河南省工程实验室,河南新乡453000 [2]郑州大学,河南郑州450000

出　　处：《动物医学进展》2022年第12期84-89,共6页Progress In Veterinary Medicine

基　　金：国家重点研发计划项目(2018YFC1602900)。

摘　　要：为制备达氟沙星(DAN)多克隆抗体,建立检测DAN残留的间接竞争ELISA(ic-ELISA)方法,采用混合酸酐法制备免疫原DAN-BSA和包被原DAN-OVA,用DAN-BSA免疫新西兰大白兔获得DAN多抗血清,建立检测DAN的ic-ELISA检测方法。结果显示,成功合成了DAN-BSA和DAN-OVA,并获得DAN的多克隆抗体;用该抗体建立的ic-ELISA检测方法的半数抑制浓度为0.39μg/L,最低检测限为0.04μg/L,检测范围为0.09μg/L~6.16μg/L。结果表明,成功制备了抗DAN的多克隆抗体,并建立了检测DAN的ic-ELISA方法,为进一步开发DAN快速检测试剂盒奠定了基础。To prepare danofloxacin(DAN)polyclonal antibodies and establish an ic-ELISA method for detecting DAN residue,the immunogen DAN-BSA and the coating original DAN-OVA were prepared by the mixed acid anhydride method.The polyclonal antibodies were obtained by immunizing New Zealand white rabbits with DAN-BSA,and an ic-ELISA to detect DAN was established.The results showed that DAN-BSA and DAN-OVA were successfully synthesized,and DAN polyclonal antibodies were successfully obtained;The half inhibitory concentration of the ic-ELISA method is 0.39μg/L,the lowest detection limit is 0.04μg/L,and the detection range is 0.09μg/L-6.16μg/L.In summary,the ic-ELISA method was established with polyclonal antibodies for detecting DAN,which laid the foundation for the further development of DAN rapid detection kit.

关 键 词：达氟沙星 完全抗原 多克隆抗体 间接竞争酶联免疫吸附试验 

分 类 号：S859.7[农业科学—临床兽医学]