玛卡调控5-HT、DA对小鼠自闭症样行为及神经环路的影响  

Effects of Maca by Regulating 5-HT and DA on Autism-like Behavior and Neural Circuit in Mice

作　　者：罗舒心 刘仲钰 梁明坤[2] 钟振国[1] 钟静 LUO Shuxin;LIU Zhongyu;LIANG Mingkun;ZHONG Zhenguo;ZHONG Jing(Scientific Experimental Center of Guangxi University of Chinese Medicine,Nanning,Guangxi,530200,China;Neurology Department,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi,530011,China;School of Basic Medicine of Guangxi University of Chinese Medicine,Nanning,Guangxi,530200,China;Postdoctoral Workstation,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning,Guangxi,530023,China)

机构地区：[1]广西中医药大学科学实验中心,广西南宁530200 [2]广西中医药大学附属瑞康医院神经内科,广西南宁530011 [3]广西中医药大学基础医学院,广西南宁530200 [4]广西中医药大学第一附属医院博士后工作站,广西南宁530023

出　　处：《广西科学》2022年第5期959-970,共12页Guangxi Sciences

基　　金：国家自然科学基金项目(81960807);广西自然科学基金青年科学基金项目(2018GXNSFBA138008);广西高校引进海外高层次人才“百人计划”;2017年广西高校中青年教师基础能力提升项目(2017KY0283);广西自然科学基金面上项目(2018GXNSFAA281225);广西中医药大学高层次人才队伍建设三年行动计划“桂派杏林青年英才”项目资助。

摘　　要：通过研究玛卡调控5-羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(Dopamine,DA)对小鼠自闭症样行为及相关神经环路的影响,初步探讨玛卡治疗自闭症的疗效及其作用机制。在整个实验中将ICR小鼠及C57/BL6小鼠分别随机设空白对照组及玛卡低、中、高剂量组,采用灌胃给药的方式,并通过高架十字迷宫实验、旷场实验及三腔社交实验,观察玛卡给药后两种不同种系小鼠行为学的变化;采用酶联免疫试剂盒检测小鼠血浆5-HT及DA含量;通过c-fos蛋白检测相关脑区的神经元活性。结果显示,与空白对照组比较,玛卡提高了ICR小鼠在高架十字迷宫实验中进入开放臂的时间、次数及指数(P<0.05或P<0.01),减少了其在旷场实验中的总移动距离(P<0.05或P<0.01),提高了其在三腔社交实验中的社会性指数、社会新颖性指数与社会记忆指数(P<0.05),并提高了其血浆5-HT及DA含量(P<0.05或P<0.01或P<0.001);c-fos检测结果显示,VTA、VP、NAcc、mPOA及amygdala阳性细胞数目明显增多(P<0.05或P<0.01或P<0.001)。本研究结果提示,玛卡对ICR小鼠自闭症样行为有明显的改善作用,且该作用可能是通过调节5-HT及DA的水平并影响相关的神经环路实现的。By studying the effects of Maca regulating 5-hydroxytryptamine(5-HT)and dopamine(DA)on autism-like behavior and related neural circuits in mice,the efficacy and mechanism of Maca in the treatment of autism were preliminarily discussed.In the whole experiment,ICR mice and C57/BL6 mice were randomly divided into blank control group and Maca low-,medium-and high-dose groups.The drug was administered by intragastric gavage,and the elevated cross labyrinth test,open field test and three-chamber social test were carried out to observe the behavioral changes of two different strains of mice after Maca administration.The contents of 5-HT and DA in plasma of mice were detected by enzyme-linked immunosorbent assay kit.The activity of neurons in related brain regions was detected by c-fos protein.The results showed compare with the blank control group,Maca increased the time,times and index of entering the open arm in the elevated cross labyrinth test of ICR mice(P<0.05 or P<0.01),decreased the total distance in the open field test(P<0.05 or P<0.01),and increased the social index,social novelty index and social memory index in the three chambers test(P<0.05).The contents of 5-HT and DA in plasma were increased(P<0.05 or P<0.01 or P<0.001).The results of c-fos detection showed that the number of positive cells in VTA,VP,NAcc,mPOA and amygdala increased significantly(P<0.05 or P<0.01 or P<0.001).The results of this study suggest that Maca has a significant improvement effect on autism-like behavior in ICR mice,and this effect may be achieved by regulating the levels of 5-HT and DA and affecting the related neural circuits.

关 键 词：玛卡 自闭症 社会行为 5-羟色胺 多巴胺 社会脑 机制 

分 类 号：R965[医药卫生—药理学]