Electrically controlled mRNA delivery using a polypyrrolegraphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells  

作　　者：Huijung Kim Kübra Solak Yoojoong Han Yeon-Woo Cho Kyeong-Mo Koo Chang-Dae Kim Zhengtang Luo Hyungbin Son Hyung-Ryong Kim Ahmet Mavi Tae-Hyung Kim 

机构地区：[1]School of Integrative Engineering,Chung-Ang University,84 Heukseuk-ro,Dongjak-gu,Seoul 06974,Republic of Korea [2]Department of Nanoscience and Nanoengineering,Graduate School of Natural and Applied Sciences,Atatürk University,Erzurum 25240,Turkey [3]Department of Chemical and Biological Engineering,Hong Kong University of Science and Technology,Kowloon,Hong Kong 999077,China [4]Department of Pharmacology,College of Dentistry,Jeonbuk National University,Jeonju 54896,Republic of Korea [5]Department of Nanoscience and Nanoengineering,Institute of Science,Atatürk University,Erzurum 25240,Turkey [6]Department of Mathematics and Science Education,Education Faculty of Kazım Karabekir,Atatürk University,Erzurum 25240,Turkey

出　　处：《Nano Research》2022年第10期9253-9263,共11页纳米研究（英文版）

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(Nos.NRF-2019M3A9H_(2)031820,NRF-2021R1A2C1010747,and NRF-2022R1A2C4002217);Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT,and Ministry of Health and Welfare(Grant No.RS-2022-00070316);the Bio&Medical Technology Development Program funded by the Ministry of Science,ICT and Future Planning,Republic of Korea(NRF-2017M3A9E4047243);d K.S.also thankful for The Council of Higher Education(CoHE,100/2000)PhD Scholarship Program,Turkey.

摘　　要：Direct messenger ribonucleic acid(mRNA)delivery to target cells or tissues has revolutionized the field of biotechnology.However,the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers.Herein,we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells(hADMSCs)via electrically controlled mRNA delivery.To find optimal electrical conductivity and mRNAloading capacity,the polypyrrole-graphene oxide(PPy-GO)hybrid film was electropolymerized on indium tin oxide substrates.We found that the fluorescein sodium salt,a molecule partially mimicking the physical and chemical properties of mRNAs,can be effectively absorbed and released by electrical stimulation(ES).The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation.This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.

关 键 词：messenger ribonucleic acid(mRNA)delivery POLYPYRROLE graphene oxide electrical release mesenchymal stem cells 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] TQ127.11[医药卫生—基础医学]