生长素信号通路参与细菌群体感应信号分子DSF对拟南芥根生长的调控  

Auxin signaling pathway is involved in the regulation of root growth by bacterial quorum sensing molecule DSF in Arabidopsis thaliana

作　　者：翟婷婷 刘方 赵芊[2,3] 何子玮 蔡硕珍 贾振华 宋水山[2,3] 吕志堂 ZHAI Tingting;LIU Fang;ZHAO Qian;HE Ziwei;CAI Shuozhen;JIA Zhenhua;SONG Shuishan;LU Zhitang(College of Life Sciences,Hebei University,Institute of Life Science and Green Development,Baoding,Hebei 071002,China;Biology Insitute,Hebei Academy of Sciences,Shijiazhuang 050051,China;Hebei Technology Innovation Center of Microbiological Control on Main Crop Disease,Shijiazhuang 050051,China;Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041,China)

机构地区：[1]河北大学生命科学学院,生命科学与绿色发展研究院,河北保定071002 [2]河北省科学院生物研究所,石家庄050051 [3]河北省主要农作物病害微生物控制技术创新中心,石家庄050051 [4]石家庄市农林科学研究院,石家庄050041

出　　处：《植物生理学报》2022年第9期1724-1734,共11页Plant Physiology Journal

基　　金：河北省中央引导地方科技发展资金项目(226Z6501G);国家自然科学基金(31601144);国家重点基础研究计划(2015CB150600)。

摘　　要：DSF(diffusible signaling factor)是革兰氏阴性细菌产生的群体感应信号分子,以浓度依赖的方式调节细菌的群体行为。近年来有研究表明,植物可以感应细菌DSF信号调节其自身免疫反应,然而,细菌DSF信号调控植物生长的作用及其作用机制尚不清楚。本研究分析了不同浓度DSF对拟南芥根系生长的影响,初步探讨了DSF促进拟南芥主根伸长的作用机制。结果显示,2μmol·L^(-1) DSF显著促进拟南芥主根伸长,增加侧根和根原基密度,并通过促进根尖分生区细胞增殖和伸长区细胞伸长实现其促进主根伸长的效应;DSF显著诱导植物体内生长素合成、转运及调控相关基因YUC7、PIN3、ARF5和ARF7的表达,增强生长素在根尖细胞的积累和极性运输;生长素合成功能缺失突变体yuc7的根系生长对DSF处理不敏感。以上结果表明,DSF可能通过调控生长素信号促进拟南芥根的生长。该研究结果有助于深入解析植物和微生物之间的跨界通讯机制。DSF(diffusible signaling factor)is a quorum-sensing signaling molecule produced by Gram-negative bacteria,which regulates the quorum behavior of bacteria in a concentration-dependent manner.In recent years,studies have shown that plants can sense bacterial DSF signals to regulate autoimmune responses.However,the effects of bacterial DSF signals on plant growth and their acting mechanisms remains largely unknown.In the present research,the effects of different concentrations of DSF on Arabidopsis thaliana root growth were analyzed,and the mechanism of Arabidopsis thaliana primary root elongation promoted by DSF was preliminarily discussed.The results showed that 2μmol·L^(-1) DSF could significantly promote the elongation of the primary root,increase the density of lateral roots and root primordia in Arabidopsis thaliana.Promotion of primary root elongation by DSF was due to the enhancement of cell division in the meristem zone and cell expansion in the elongation zone.DSF significantly increased the expression of gene involved in auxin synthesis,transportation and regulation,such as YUC7,PIN3,ARF5 and ARF7.DSF enhanced the accumulation and polar transportation of auxin in Arabidopsis thaliana root cells.Auxin synthesis defective mutant yuc7 is insensitive to DSF treatment with respect to root growth.Taken togerther,the results suggest that DSF may enhance the root growth via auxin signaling in Arabidopsis thaliana.It is helpful in elucidating the mechanism of cross-kingdom communication between plants and microorganisms.

关 键 词：DSF 拟南芥 根的生长 生长素 

分 类 号：Q945[生物学—植物学]