生长光强对黄瓜叶片光合机构活性影响研究  

Effects of growth light intensity on the activity of photosystem in cucumber leaves

作　　者：罗蛟 丁新宇 徐燕妮 王俊彦 张强 孙山[2] 李玉婷 高辉远[1] 张子山[1] LUO Jiao;DING Xinyu;XU Yanni;WANG Junyan;ZHANG Qiang;SUN Shan;LI Yuting;GAO Huiyuan;ZHANG Zishan(College of Life Sciences,Shandong Agricultural University,State Key Laboratory of Crop Biology,Taian,Shandong 271018,China;Shandong Institute of Pomology,Taian,Shandong 271099,China;College of Agronomy,Shandong Agricultural University,Taian,Shandong 271018,China)

机构地区：[1]山东农业大学生命科学学院,作物生物学国家重点实验室,山东泰安271018 [2]山东省果树研究所,山东泰安271099 [3]山东农业大学农学院,山东泰安271018

出　　处：《植物生理学报》2022年第9期1790-1800,共11页Plant Physiology Journal

基　　金：国家自然科学基金(31771691);山东省自然科学基金青年基金(ZR2017QC001和ZR2020QC104)。

摘　　要：本研究利用快速叶绿素荧光诱导动力学曲线(OJIP曲线)和820 nm光吸收技术,系统研究了生长光强(50、100、200μmol·m^(-2)·s^(-1))对黄瓜(Cucumis sativus)叶片光系统Ⅱ(PSⅡ)和光系统Ⅰ(PSⅠ)原初光化学反应活性及能量传递效率的影响。随着生长光强增加,OJIP曲线的K、J、I三个特征点数值均明显下降,L点没有明显变化,这表明,生长光强对类囊体的垛叠没有明显影响,但改善了PSⅡ供体侧和受体侧的能量传递效率,其中天线吸收光能能力减弱,能量捕获效率不受影响,电子传递效率明显增加。生长光强的增加同时影响了PSⅡ天线的能量吸收和反应中心活性,但只提高了PSⅠ有活性反应中心的密度而没有影响天线大小。强光生长下,黄瓜叶片质体醌含量更大,可以向下游传递更多电子,从而使反应中心重新开放。本研究表明,生长光强小幅变化即对黄瓜叶片光能吸收和能量传递效率造成明显影响;OJIP曲线和820 nm光吸收技术是在光合机构未受伤害时比较不同植物叶片光合原初光化学反应活性和能量传递效率的有效手段。In this study,the effects of growth light intensity(50,100,and 200μmol·m^(-2)·s^(-1))on the primary photochemical reaction activity and energy transfer efficiency of photosystem Ⅱ(PSⅡ)and photosystem Ⅰ(PSⅠ)in leaves of cucumber(Cucumis sativus)seedling were systematically studied by using chlorophyll a fluorescence transient(OJIP curve)and 820 nm light absorption technology.The increase of growth light intensity decreased the K,J and I steps of OJIP curve,but did not change the L step,indicating that the growth light intensity had no significant effect on the stacking of thylakoids,but improved the energy transfer efficiency at donor side and receptor side of PSⅡ.With the increase of growth light intensity,the ability of PSⅡ antenna to absorb light energy was weakened,the energy capture efficiency of reaction center was not affected,and the efficiency of electron transfer was increased significantly.The increase of growth light intensity affected both the energy absorption of PSⅡ antenna and the activity of reaction center,but only improved the density of active reaction center of PSⅠ without affecting the size of antenna.The plastid quinone content of cucumber leaves grown under higher light is more,which can transfer more electrons to the downstream,so as to reopen the reaction center.This study proves that a small change of growth light intensity has a significant impact on the primary photochemical reaction activity and energy transfer efficiency in cucumber leaves,and the OJIP curve and 820 nm light absorption technology are effective tools to compare the primary photosynthetic photochemical reaction activity and energy transfer efficiency in different plants when the photosynthetic mechanism was not damaged.

关 键 词：生长光强 快速叶绿素荧光诱导动力学曲线 820 nm光吸收 光系统Ⅰ 光系统Ⅱ 

分 类 号：S642.2[农业科学—蔬菜学]