Self-powered anti-interference photoelectrochemical immunosensor based on Au/ZIS/CIS heterojunction photocathode with zwitterionic peptide anchoring  

作　　者：Ze Hu Yaqun Xu Hao Wang Gao-Chao Fan Xiliang Luo 

机构地区：[1]Shandong Key Laboratory of Biochemical Analysis,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China [2]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China

出　　处：《Chinese Chemical Letters》2022年第11期4750-4755,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22074073,21275087);the Natural Science Foundation of Shandong Province of China(No.ZR2021YQ11);the Taishan Scholar Program of Shandong Province of China(No.ts20110829)。

摘　　要：Accurate detection of important biomarkers with ultra-low levels in complex biological matrix is one of the frontier scientific issues because of possible signal interference of potential reductive agents and protein molecules.Herein,a self-powered anti-interference photoelectrochemical(PEC)immunosensor was explored for sensitive and specific detection of model target of cardiac troponinⅠ(cTnI).Specifically,a novel ternary heterojunction served as the photocathode to offer a remarkable current output and a zwitterionic peptide was introduced to build a robust antifouling biointerface.CuInS(CIS)film with porous network nanostructure was first prepared and then modified in order with ZnInS(ZIS)nanocrystals and Au nanoparticles to fabricate the Au/ZIS/CIS heterojunction photocathode.After capture cTnI antibody(Ab)was immobilized,the zwitterionic peptide KAEAKAEAPPPPC was then anchored to compete the immunosensor.The elaborated PEC immunosensor exhibited high sensitivity for target cTnI antigen(Ag)detection,with good anti-interference against reductive agents and nonspecific proteins.This integration strategy of heterojunction photocathode with zwitterionic peptide provides a new sight to develop advanced PEC immunosensors applying in practical biosamples.

关 键 词：PHOTOELECTROCHEMISTRY Immunoassay Zwitterionic peptide PHOTOCATHODE ANTI-INTERFERENCE 

分 类 号：R392[医药卫生—免疫学] O657.1[医药卫生—基础医学] TP212[理学—分析化学]