光皮桦AP2/ERF基因家族鉴定与表达分析  

Identification and expression analysis of AP2/ERF gene family in Betula luminifera

作　　者：黄奕孜 钱旺 邱姗 王文新 黄华宏[1] 林二培[1] HUANG Yizi;QIAN Wang;QIU Shan;WANG Wenxin;HUANG Huahong;LIN Erpei(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2022年第6期1183-1193,共11页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31770641);浙江省重点研发计划项目(2021C02037)。

摘　　要：【目的】深入研究AP2/ERF基因家族在光皮桦Betula luminifera生长发育及环境胁迫响应中的生物学功能。【方法】利用光皮桦基因组数据,通过生物信息学方法开展AP2/ERF基因家族鉴定、基因特征、系统进化、基因结构、保守基序、顺式作用元件、蛋白互作和表达分析。【结果】在光皮桦基因组中共鉴定到77个AP2/ERF基因,其编码的蛋白理化性质存在差异,大多数蛋白(60个)的理论等电点小于7.0。系统进化分析显示:这77个AP2/ERF转录因子属于5个亚家族,其中ERF亚家族最大,包含34个成员。光皮桦AP2/ERF各亚家族间的基因结构存在较大差异,其中AP2亚家族成员均具有6~9个内含子,而DREB亚家族基因则没有内含子;但AP2/ERF各亚家族内的不同成员具有相似的保守基序类型和分布。同时,AP2/ERF基因启动子上都存在大量与激素、调节、胁迫响应及生长发育相关的顺式作用元件。此外,互作网络分析预测不同的光皮桦AP2/ERF亚家族蛋白间存在广泛的互作关系。进一步的表达分析显示:绝大多数光皮桦AP2/ERF基因(71个)的表达存在较强组织特异性,且在高温胁迫下,多数ERF或DREB基因的表达发生显著变化,表明ERF和DREB基因在高温胁迫应答中可能发挥着重要作用。【结论】通过生物信息学分析获得光皮桦77个AP2/ERF基因,分属于5个亚家族。不同亚家族基因具有相似的基因结构、保守基序等特征。基因启动子区含激素、胁迫响应等相关的作用元件。基因表达具有较强的组织特异性,且多数ERF或DREB基因对高温胁迫有明显响应。图6表1参39。[Objective] This study aims to explore biological roles of AP2/ERF gene family in growth and development of Betula luminifera, and its responses to environmental stress. [Method] Based on the genome data of B. luminifera, AP2/ERF gene family were identified through bioinformatics method, and their gene features, phylogeny, gene structure, conserved motifs, cis-acting elements, protein interactions and expression pattern were analyzed. [Result] A total of 77 AP2/ERF genes were identified in the genome of B. luminifera.The physical and chemical properties of the encoded proteins were different, and the isoelectric points of most proteins(60) were less than 7.0. Phylogenetic analysis showed that these 77 AP2/ERF transcription factors belonged to 5 subfamilies, among which ERF subfamily was the largest, including 34 members. The gene structures of AP2/ERF subfamilies were quite different. The members of AP2 subfamily had 6-9 introns, while DREB subfamily gene had no introns. However, similar conserved motif types and distributions were observed in different members of AP2/ERF subfamilies. At the same time, there were a large number of cis acting elements related to hormone, regulation, stress responses, and growth and development in promoter of AP2/ERF genes. Protein interaction network analysis predicted that there were extensive interactions among different AP2/ERF subfamily proteins. Further expressions analysis showed that the expression of most AP2/ERF genes(71) had strong tissue specificity, and the expressions of most ERF or DREB genes changed significantly under heat stress, indicating that ERF and DREB genes might play an important role in the response to heat stress.[Conclusion] Through bioinformatics analysis, 77 AP2/ERF genes are identified in B. luminifera, which belong to 5 subfamilies. Different subfamily genes have similar gene structure and conserved motifs. The promoter region of the gene contains hormone, stress response and other related elements. Gene expression has strong tissue specificity, and

关 键 词：光皮桦 AP2/ERF家族 表达模式 高温胁迫 

分 类 号：S722.3[农业科学—林木遗传育种]