不同生长阶段呼伦贝尔羊3种脂肪组织中膻味脂肪酸含量的变化规律研究  

Change Law of Odor Fatty Acid Contents in Three Adipose Tissues of Hulun Buir Sheep at Different Growth Stages

作　　者：欧慧敏 张小丽 钟荣珍[3] 李建中[2] 谭支良[1] 许丽卫[1] 焦金真[1] OU Huimin;ZHANG Xiaoli;ZHONG Rongzhen;LI Jianzhong;TAN Zhiliang;XU Liwei;JIAO Jinzhen(National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,CAS Key Laboratory of Agroecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation,College of Life Sciences,Hunan Normal University,Changsha 410081,China;Jilin Provincial Key Laboratory of Grassland Farming,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,长沙410125 [2]湖南师范大学生命科学学院,动物肠道功能湖南省重点实验室,长沙410081 [3]中国科学院东北地理与农业生态研究所,吉林省草地畜牧重点实验室,长春130102

出　　处：《动物营养学报》2022年第11期7220-7227,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：中国科学院战略性先导专项(A类)子课题(XDA26040305)。

摘　　要：本试验旨在研究不同生长阶段的呼伦贝尔羊脂肪组织中膻味脂肪酸含量的变化规律。以新生期、断奶期、育肥中期和后期4个不同生长阶段的32只呼伦贝尔羊为研究对象,采集背部皮下脂肪、肾周脂肪和肠系膜脂肪组织样品,采用索式抽提法收集脂肪后进行酸碱法甲酯化处理,并联合使用气相色谱-质谱分析,对4-甲基辛酸(MOA)、4-乙基辛酸(EOA)和4-甲基壬酸(MNA)的含量进行了测定和比较分析。结果表明:1)MOA、EOA、MNA的定量检测限分别为0.10、0.05和0.05μg/mL,线性相关系数(R~2)>0.998。2)呼伦贝尔羊背部皮下脂肪和肾周脂肪组织中MOA、EOA和MNA的含量随生长阶段的变化呈显著差异(P<0.05),均表现为育肥后期>育肥中期>断奶期>新生期,且在断奶期~育肥中期的增长速率最快。在肠系膜脂肪组织中,新生期的3种膻味脂肪酸含量低于检测线,而育肥中期和后期的MOA和EOA的含量均显著高于断奶期(P<0.05)。3)膻味脂肪酸的含量存在部位差异性,即背部皮下脂肪组织高于肠系膜和肾周脂肪组织。上述结果表明,呼伦贝尔羊脂肪组织中膻味脂肪酸含量在断奶期至育肥中期的增长速率最快,且以背部皮下脂肪组织中含量最高。This experiment aimed to study the change law of the contents of odor fatty acids in three adipose tissues at different growth stages of Hulun Buir sheep.Thirty-two Hulun Buir sheep at four different growth stages(neonatal stage,weaning stage,mid-fattening stage and late-fattening stage)were used in this study.The dorsa subcutaneous,perirenal and mesenteric adipose tissue samples were collected.After the fat was extracted from the adipose tissue using the Soxhlet extraction method,the fatty acids were methylated by acid-alkali method and then analyzed with gas chromatography-mass spectrometry(GC-MS).The contents of 4-methyloctanoic acid(MOA),4-ethyloctanoic acid(EOA)and 4-methylnonanoic acid(MNA)were quantitatively determined and compared among different growth stages.The results showed as follows:1)the quantitative detection limits of MOA,EOA and MNA were 0.10,0.05 and 0.05μg/mL,respectively,and the linear correlation coefficients(R~2)were larger than 0.998.2)In the dorsa subcutaneous and perirenal adipose tissues,the contents of MOA,EOA and MNA were significantly different among four growth stages(late-fattening stage>mid-fattening stage>weaning stage>neonatal stage,P<0.05).The most rapid change occurred between weaning and mid-fattening stages.In mesenteric adipose tissue,the contents of three odor fatty acids in neonatal stage were lower than the detection line,and the contents of MOA and EOA in mid-fattening and late-fattening stages were significantly higher than those in weaning sheep(P<0.05).3)The contents of odor fatty acids in dorsa subcutaneous adipose tissue were higher than mesenteric and perirenal adipose tissue.In summary,the deposition rate of odor fatty acids of Hulun Buir sheep is the fastest between the weaning and the midfattening stages,and the dorsa subcutaneous adipose tissue has the highest odor fatty acids among three adipose tissues.[Chinese Journal of Animal Nutrition,2022,34(11):7220-7227]

关 键 词：不同生长阶段 呼伦贝尔羊 脂肪组织 膻味脂肪酸 

分 类 号：S826[农业科学—畜牧学]