黄连生物碱对HEK293-OATP1B3细胞转运黄芩黄酮活性的影响  

Influence of Coptis chinensis Alkaloid on the Activity of HEK293-OATP1B3 Cells in Transport of Scutellaria baicalensis Flavones

作　　者：梁燕 崔馨戈 訾苗苗 汪杰 刘艳 王茜 彭灿 LIANG Yan;CUI Xin-ge;ZI Miaomiao;WANG Jie;LIU Yan;WANG Qian;PENG Can(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Key Laboratory of Chinese Medicinal Formula of Anhui Province,Anhui Hefei 230012,China;Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application,Anhui Hefei 230012,China;Institute of Traditional Chinese Medicine Resources Protection and Development,Anhui Academy of Chinese Medicine,Anhui Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中药复方安徽省重点实验室,安徽合肥230012 [3]药物制剂技术与应用安徽省重点实验室,安徽合肥230012 [4]安徽省中医药研究院中药资源保护与开发研究所,安徽合肥230012

出　　处：《安徽中医药大学学报》2022年第6期89-93,共5页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(8180140889);安徽省协同创新项目(GXXT-2020-025)。

摘　　要：目的探究黄连生物碱对HEK293-OATP1B3介导的黄芩苷、汉黄芩苷转运的影响。方法采用稳定转染OATP1B3的HEK293细胞,以黄芩苷和汉黄芩苷为底物,小檗碱、药根碱、黄连碱、盐酸巴马汀作为抑制剂,进行药物转运实验,并进行含量测定。结果抑制剂组的黄芩苷、汉黄芩苷摄取量均低于对照组。结论黄连生物碱可抑制OATP1B3对黄芩苷和汉黄芩苷的转运。Objective To investigate the influence of Coptis chinensis alkaloid on the transport of baicalin and wogonoside mediated by OATP1B3.Methods HEK293 cells stably transfected with OATP1B3 were used to conduct a drug transport study,with baicalin and wogonoside as the substrates and berberine,jatrorrhizine,coptisine,and palmatine chloride as the inhibitors,and content determination was also performed.Results The inhibitor group had a significantly lower uptake of baicalin and wogonoside than the control group.Conclusion Coptis chinensis alkaloid can inhibit the transport of baicalin and wogonoside mediated by OATP1B3.

关 键 词：黄连生物碱 黄芩苷 汉黄芩苷 OATP1B3 转运 

分 类 号：R285.5[医药卫生—中药学]