基于苄基三乙基溴化铵和正辛酸的低共熔溶剂的制备及其在分散液液微萃取中的应用  

Preparation of A Deep Eutectic Solvent Based on Benzyltriethylammonium Bromide and Octanoic Acid and Its Application in Dispersion Liquid-liquid Microextraction

作　　者：葛丹丹 黄兴[2] 马兴娅 石毓秀 GE Dan-dan;HUANG Xing;MA Xing-ya;SHI Yu-xiu(School of Chemistry and Chemical Engineering,Kunming University,Kunming 650214,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]昆明学院化学化工学院,云南昆明650214 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《化学试剂》2022年第12期1775-1781,共7页Chemical Reagents

基　　金：云南省高校食品安全检测技术重点实验室项目(2019);云南省地方本科高校基础研究联合专项资金项目面上项目(ZX20220116);海南省院士创新平台资金专业学位项目(2021);云南省研究生教学案例库项目建设《高等分离工程》教学案例库建设资助项目(2022)。

摘　　要：以苄基三乙基溴化铵和正辛酸制备出新型低共熔溶剂,并作为萃取溶剂建立了漩涡辅助分散液-液微萃取结合高效液相色谱测定3种苏丹红染料的方法。对影响萃取效率的各种参数,包括低共熔溶剂的组成、萃取时间、萃取剂用量、溶液pH值和盐浓度等进行了优化。结果表明最佳萃取条件为:以苄基三乙基溴化铵与正辛酸物质的量比1∶2制备出的低共熔溶剂为萃取溶剂,萃取剂用量为50μL,萃漩涡时间为2 min,溶液pH 7,盐浓度为0 mg/mL。在最优化条件下,3种苏丹红染料在8~1000 ng/mL范围内线性关系良好,相关系数均大于0.9956,检出限和定量限范围分别为2~2.5 ng/mL和6~8 ng/mL,对果汁和环境水样进行加标实验,回收率为82.3%~111.5%,相对平均偏差范围为0.5%~7.2%。方法简单、快速、绿色环保,可用于真实样品中苏丹红染料的分析检测。A new hydrophobic deep eutectic solvent(DES),formed from benzyltriethylammonium bromide and octanoic acid,was used as an extraction solvent in vortex-assisted dispersive liquid-liquid microextraction combined with high-performance liquid chromatography for the preconcentration and detection of three Sudan dyes from food samples.Factors affecting the extraction efficiency including the type and volume of extraction solvent,extraction time,solution pH and salt concentration were optimized.The optimum extraction conditions were:volume of extraction solvent was 50μL,vortex time was 2 min,solution pH was 7,salt concentration was 0 mg/mL.Under the optimized condition,a linear range was in the range of 8~1000 ng/mL with a correlation coefficient higher than 0.9956,limits of detection ranged from 2 to 2.5 ng/mL,and limits of quantification from 6 to 8 ng/mL.The food samples were spiked with 20,50,and 100 ng/mL of Sudan dyes,and the extraction recoveries ranged from 82.3% to 111.5% with RSDs ranging from 0.5% to 7.2%.The developed method is simple,quick,and environmentally friendly,which can be applied to detect the Sudan dyes in real food samples.

关 键 词：低共熔溶剂 分散液-液微萃取 苏丹红染料 饮料分析 水样分析 

分 类 号：O661[理学—分析化学]