马立克氏病病毒RLORF1编码蛋白通过抑制宿主IFN-β表达促进病毒复制  

Marek’s Disease Virus RLORF1-encoded Protein Facilitates Virus Replication by Inhibiting Host IFN-β Expression

作　　者：江波 蔡云虹 曹梦瑶 刘文晓 程晶 许健[1,3] 李永清 JIANG Bo;CAI Yun-hong;CAO Meng-yao;LIU Wen-xiao;CHENG Jing;XU Jian;LI Yong-qing(Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京海淀100097 [2]北京农学院动物科学技术学院,北京昌平102206 [3]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《中国兽医杂志》2022年第10期8-14,共7页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金青年基金项目(31902245);北京市农林科学院科技创新能力建设专项(KJCX20220422)。

摘　　要：为了研究马立克氏病病毒(MDV)抑制宿主天然免疫的机制,本试验构建了RLORF 1基因缺失MDV(CVI988^(△RLORF1)和RB1B^(△RLORF1)),结合RLORF 1基因的过表达方法探讨该基因对MDV复制以及Ⅰ型干扰素反应的影响。结果显示,MDV RLORF 1基因缺失一方面降低了病毒复制能力(P<0.001),另一方面上调了病毒诱导宿主的Ⅰ型干扰素反应的能力(P<0.05或P<0.001),而在鸡胚成纤维细胞(CEF)中过表达RLORF 1后,MDV病毒的复制能力明显增强(P<0.001),且RLORF 1基因转录蛋白可显著抑制宿主的干扰素-β(IFN-β)表达(P<0.001)。结果表明,MDV感染过程中可以通过RLORF 1基因编码蛋白抑制宿主的Ⅰ型干扰素表达,进而通过抑制宿主的天然免疫反应保证病毒感染后的早期复制。In order to investigate the mechanism of Marek's disease virus(MDV)inhibiting host innate immunity,this study utilized MDV deleted RLORF 1(CVI988^(△RLORF1) and RB1B^(△RLORF1))in combination with eukaryotic expression plasmid(PEGF-N1-RLORF1)to determine the effect of RLORF 1 gene on the replication of MDV and the role of RLORF 1 gene in the regulation of host type I interferon signaling pathway.The results showed that MDV deleted RLORF 1 could decrease viral replication(P<0.001),and up-regulate the ability of virus to induce host type I interferon response(P<0.05 or P<0.001).The same result was obtained in the gain-function experiment that the replication ability of MDV was significantly enhanced in CEF cells with RLORF 1 overexpression(P<0.001).Moreover,RLORF 1 gene played a role in inhibiting host IFN-βtranscription during MDV infection(P<0.001).Thus,the RLORF 1 gene encoding protein could inhibit the pathway of type I interferon in the host during MDV infection,and ensure the early replication of the virus after infection.

关 键 词：马立克氏病病毒 RLORF1基因 Ⅰ型干扰素 病毒复制 

分 类 号：S852.659.3[农业科学—基础兽医学]