群体感应淬灭酶对MBR-CANON工艺脱氮性能及污泥特性的影响  

Effect of quorum quenching enzyme on the nitrogen removal performance and sludge property of MBR-CANON process

作　　者：张肖静 张红丽 张楠 位登辉 马冰冰 张涵 杨浩洁 ZHANG Xiaojing;ZHANG Hongli;ZHANG Nan;WEI Denghui;MA Bingbing;ZHANG Han;YANG Haojie(He′nan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration,Zhengzhou University of Light Industry,Zhengzhou 450001,China)

机构地区：[1]郑州轻工业大学环境污染治理与生态修复河南省协同创新中心,河南郑州450001

出　　处：《轻工学报》2022年第6期110-118,共9页Journal of Light Industry

基　　金：国家自然科学基金项目(41701569);河南省高校科技创新人才支持计划项目(20HASTIT014)。

摘　　要：在稳定运行的基于膜生物反应器的全程自养脱氮工艺过程中投加淬灭酶-酰基高丝氨酸内酯(Acyl-Homoserine-Lactones,AHLs)酰基转移酶,跟踪分析该工艺过程中的脱氮性能、污泥性能、关键酶活及功能微生物组成的变化情况。结果表明,AHLs酰基转移酶的加入导致好氧氨氧化菌的活性严重下降,当AHLs酰基转移酶质量浓度为0.5 mg/L时,氨氮去除率从最初的83.7%下降至57.0%,总氮去除率由65.9%下降至43.6%,但AHLs酰基转移酶可大幅降低膜丝表面附着的污泥中胞外聚合物含量,有利于减缓膜污染;AHLs酰基转移酶的加入导致自养脱氮关键酶—羟氨氧化还原酶、氨单加氧酶及亚硝酸盐还原酶的活性下降。AHLs酰基转移酶对好氧氨氧化菌的活性抑制大于厌氧氨氧化菌,但对两者相对丰度的影响则相反,厌氧氨氧化菌受到的抑制主要表现为基质不足导致的生长受限。Quorum quench enzyme-Acyl-Homoserine-Lactones(AHLs)acyltransferase was added to the stable MBR-CANON process,the nitrogen removal performance,sludge property,key enzymes′activity and microbial community were tracked and analyzed.The results showed that the addition of AHLs acyltransferase significantly suppressed the nitrogen removal ability.The AHLs acyltransferase in 0.5 mg/L obviously decreased the ammonia removal efficiency from 83.7%to 57.0%,and the total nitrogen removal efficiency from 65.9%to 43.6%.The extracellular polymeric substance in the sludge attaching to the membrane surface was also decreased by the AHLs acyltransferase,which was beneficial to alleviate the membrane fouling.Additionally,the activity of hydroxylamine oxidoreductase,ammonia monooxygenase,and nitrite reductase all significantly decreased with the addition of AHLs acyltransferase.The inhibition of AHLS acyltranferase on the activity of aerobic ammonia-oxidizing bacteria was greater than that on the anaerobic ammonia-oxidizing bacteria(AAOB),but the effect on their relative abundances was opposite.The suppression on AAOB was mainly attributed to the lack of substrate.

关 键 词：群体感应 淬灭酶 自养脱氮 胞外聚合物 膜生物反应器 关键酶活 

分 类 号：X703.1[环境科学与工程—环境工程]