Do methylenetetrahydrofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1 polymorphisms modify changes in intelligence of school-age children in areas of endemic fluorosis?  

作　　者：Zichen Feng Ning An Fangfang Yu Jun Ma Na Li Yuhui Du Meng Guo Kaihong Xu Xiangbo Hou Zhiyuan Li Guoyu Zhou Yue Ba 

机构地区：[1]Department of Occupational and Environmental Health,School of Public Health,Zhengzhou University,Zhengzhou,Henan 450001,China [2]Department of Endemic Disease,Kaifeng Center for Disease Control and Prevention,Kaifeng,Henan 475004,China [3]Dietary Center,Zhengzhou Health Vocational Cllege,Zhengzhou,Henan 450122,China

出　　处：《Chinese Medical Journal》2022年第15期1846-1854,共9页中华医学杂志（英文版）

基　　金：National Natural Science Foundation of China(Nos. 81972981, 82003401, and 81673116);Key Projects of Colleges and Universities of Henan Education Department(21A330006)。

摘　　要：Background: Excessive exposure to fluoride can reduce intelligence. Methylenetetrahydrofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1 (MTHFD1) polymorphisms have important roles in neurodevelopment. However, the association ofMTHFD1 polymorphisms with children’s intelligence changes in endemic fluorosis areas has been rarely explored.Methods: A cross-sectional study was conducted in four randomly selected primary schools in Tongxu County, Henan Province, from April to May in 2017. A total of 694 children aged 8 to 12 years were included in the study with the recruitment by the cluster sampling method. Urinary fluoride (UF) and urinary creatinine were separately determined using the fluoride ion-selective electrode and creatinine assay kit. Children were classified as the high fluoride group and control group according to the median of urinary creatinine-adjusted urinary fluoride (UFCr) level. Four loci ofMTHFD1 were genotyped, and the Combined Raven’s Test was used to evaluate children’s intelligence quotient (IQ). Generalized linear model and multinomial logistic regression model were performed to analyze the associations between children’s UFCr level,MTHFD1 polymorphisms, and intelligence. The general linear model was used to explore the effects of gene-environment and gene-gene interaction on intelligence.Results: In the high fluoride group, children’s IQ scores decreased by 2.502 when the UFCr level increased by 1.0 mg/L (β= –2.502, 95% confidence interval [CI]: –4.411, –0.593), and the possibility for having "excellent" intelligence decreased by 46.3% (odds ratio = 0.537, 95% CI: 0.290, 0.994). Children with the GG genotype showed increased IQ scores than those with the AA genotype of rs11627387 locus in the high fluoride group (P < 0.05). Interactions between fluoride exposure andMTHFD1 polymorphisms on intelligence were observed (Pinteraction < 0.05).Conclusion: Our findings suggest that excessive fluoride exposure may have adverse effects on children’s int

关 键 词：FLUORIDE INTELLIGENCE Interaction MTHFD1 gene 

分 类 号：R725.9[医药卫生—儿科]