串晶结构纤维调控成纤维细胞行为  

Shish-kebab structure fiber regulates behavior of human fibroblasts

作　　者：李晓婧 焦勇杰 李超婧 王富军[1,2,3] 王璐 LI Xiaojing;JIAO Yongjie;LI Chaojing;WANG Fujun;WANG Lu(Key Laboratory of Textile Science&Technology,Ministry of Education,Donghua University,Shanghai 201620,China;Engineering Research Center of Technical Textiles,Ministry of Education,Donghua University,Shanghai 201620,China;College of Textiles,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学纺织面料技术教育部重点实验室,上海201620 [2]东华大学产业用纺织品教育部工程研究中心,上海201620 [3]东华大学纺织学院,上海201620

出　　处：《东华大学学报（自然科学版）》2022年第6期42-49,共8页Journal of Donghua University(Natural Science)

基　　金：上海市青年科技英才扬帆计划(20YF1400800);中央高校基本科研业务费专项资金(2232020G-01);111计划2.0(BP0719035)。

摘　　要：为探究串晶(shish-kebab,SK)结构片晶密度对成纤维细胞行为的影响及其机制,结合静电纺丝技术和溶液孵育结晶法制备了表面光滑和具有不同片晶密度的串晶结构纤维支架,通过分析表面形貌、力学性能及体外细胞试验结果,探究不同拓扑结构对人皮肤成纤维细胞的形态及其增殖和基因表达的影响。结果表明,低密度的串晶结构(粗糙度为(0.85±0.10)μm)可以改善纤维支架的表面和力学性能,增大细胞铺展面积,并减少转化生长因子(TGF-β_(1))和α-平滑肌肌动蛋白(α-SMA)的表达,加快组织修复,减少瘢痕,在伤口修复中具有应用潜力。The smooth and series shish-kebab(SK)fiber scaffolds with different kebab densities were prepared by electrospinning and solution incubation methods to investigate the effect of kebab density of SK structure on the behavior of fibroblasts and its mechanism.The effects of fiber scaffolds with different surface topologies on the morphology,proliferation and gene expression of human foreskin fibroblasts were explored by analyzing the surface morphology,roughness,mechanical properties and the results of in vitro cell tests.The results show that the low-density SK structure(roughness:(0.85±0.10)μm)can improve the surface and mechanical properties of fiber scaffolds,increase cell spread area,reduce the expression of transforming growth factor-beta1(TGF-β_(1))andα-smooth muscle actin(α-SMA),accelerate wound healing and reduce scar tissues,having potential applications in wound healing.

关 键 词：串晶结构 静电纺丝 细胞行为 成纤维细胞 伤口愈合 

分 类 号：TS101.4[轻工技术与工程—纺织工程]