一株产酶真菌Phaeophlebiopsis sp.转录组分析  

Transcriptomic analysis at different growth stages of an enzyme-producing fungus Phaeophlebiopsis sp.

作　　者：程敏 郭鑫瑶 李启瑞 王迪[1,2,3] 李小兵 康颖倩 CHENG Min;GUO Xinyao;LI Qirui;WANG Di;LI Xiaobing;KANG Yingqian(Department of Microbiology,School of Basic Medicine,Guizhou Medical University,Guiyang 550025,Guizhou,China;Research Talent Base for the Relationship between Microbiology and Human Health in Guizhou Province,Key Laboratory of Pathogen Biology in Universities in Guizhou Province,Guiyang 550025,Guizhou,China;Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guiyang 550025,Guizhou,China;Guizhou Chishui Riverside Maotai-flavor Liquor Research Center,Guiyang 550003,Guizhou,China;Guizhou Academy of Tobacco Science,Guiyang 550009,Guizhou,China)

机构地区：[1]贵州医科大学基础医学院微生物学教研室,贵州贵阳550025 [2]贵州省微生物与人类健康关系研究人才基地,贵州省普通高校病原生物学特色重点实验室,贵州贵阳550025 [3]贵州医科大学环境污染与疾病监控教育部重点实验室,贵州贵阳550025 [4]贵州省赤水河畔酱酒研究中心,贵州贵阳550003 [5]贵州省烟草科学研究院,贵州贵阳550009

出　　处：《菌物学报》2022年第10期1634-1646,共13页Mycosystema

基　　金：国家自然科学基金(32060034);国家科技部及教育部高等学校学科创新引智计划(D20009);贵州省科技厅社会发展攻关支撑项目[黔科合支撑(2019)2873];贵州省科学技术厅国际科技合作基地[黔科合平台人才(2020)4101];贵州省科技计划项目[黔科合支撑(2020)4Y220];贵州省微生物与健康院士工作站([2020]4004);贵阳市科技计划项目(筑科合同[2020]-16-24)。

摘　　要：对从药渣中分离的真菌ZYJHYZ254进行鉴定及产酶活性研究,从转录组分析菌株不同生长时期差异表达基因对其生长发育及产酶调控的影响,以筛选高产木质纤维素水解酶真菌,寻找调控关键基因。鉴定ZYJHYZ254为拟暗射脉菌Phaeophlebiopsissp.,产酶在第5–7天最高。从生长3 d与7 d的菌丝中共检测到1232个差异基因,以3 d的菌丝为对照,显著上调、下调基因分别有826及406个,基因注释和GO、KEGG功能富集分析结果表明差异表达基因主要与蛋白质合成、代谢及酶合成相关。此外,共有387个CAZymes基因表达,GH数量最多,约占49.61%,其次为AA(97)与GT(62),约占25.06%与16.02%。GH16(24个)占GH的12.50%,含量最多,主要编码葡萄糖苷酶、木聚糖酶等,AA中AA3(37个)占比38.14%,编码氧化酶、脱氢酶等。结果表明ZYJHYZ254中生长3 d与7 d的菌丝经功能富集分析发现差异表达基因主要与蛋白质合成、代谢,以及酶合成相关。进一步研究发现在两个生长时期中CAZymes基因表达最多的是GH16与AA3,预示了该菌葡萄糖苷酶、木聚糖酶、β-半乳糖苷酶、氧化酶与脱氢酶含量最丰富,对降解特殊生物质中的木质纤维素具有重要意义。A fungal strain ZYJHYZ254 isolated from medicine residues was identified as Phaeophlebiopsis sp.and its enzyme production activity was studied.The effects of differentially expressed genes on the growth and development of ZYJHYZ254 at different growth stages were analyzed on the basis of transcriptome to obtain high yield strain producing lignocellulosic hydrolase and search for related key regulation genes.It showed that cellulase production of ZYJHYZ254 peaked on the 5day,and xylanase and lignin peroxidase production peaked on the 7day of fermentation.A total of 1232 differentially expressed genes was detected in mycelia aged 3 and 7 d.Taking 3 d-old mycelia as the control,826 and 406 genes were significantly up-regulated and down-regulated,respectively.Gene annotation and GO and KEGG functional enrichment analysis showed that differentially expressed genes were mainly related to protein synthesis,metabolism and enzyme synthesis.In addition,a total of 387 CAZymes genes was expressed,and GH genes were the most abundant,accounting for 49.61%,and AA(97)and GT(62)ranked second and third,accounting for 25.06%and 16.02%,respectively.GH16(24),accounting for 12.50%of GH,is the most abundant in GH,mainly encoding glucosidase,xylanase,etc.AA3(37)is the most abundant in AA,accounting for 38.14%,encoding oxidase,dehydrogenase,etc.Further study found that GH16 and AA3 were most expressed in CAZymes in 3 d-old and 7 d-old mycelia,indicating that this strain was rich in glucosidase,xylanase,β-galactosidase,oxidase and dehydrogenase,which was of great significance for the degradation of lignocellulose in special biomass.

关 键 词：特殊环境 丝状真菌 转录组学 木质纤维素 碳水化合物活性酶 

分 类 号：Q933[生物学—微生物学]