精胺合成酶MrSPS参与调控罗伯茨绿僵菌的生长发育、抗逆和致病力  

Spermine synthase is involved in the regulation of development,stress response and pathogenicity of Metarhizium robertsii

作　　者：伍君瑶 李心怡 宛文倩 肖硕 陆陈丽 秦丽[1] 黄勃[1] 王玉龙[1] WU Junyao;LI Xinyi;WAN Wenqian;XIAO Shuo;LU Chenli;QIN Li;HUANG Bo;WANG Yulong(Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036,Anhui,China)

机构地区：[1]安徽农业大学微生物防治省重点实验室,安徽合肥230036

出　　处：《菌物学报》2022年第10期1647-1657,共11页Mycosystema

基　　金：中国博士后科学基金项目(2020M671838);安徽省大学生创新训练计划(S202010364088,S202110364189)。

摘　　要：本研究以罗伯茨绿僵菌Metarhizium robertsii为研究对象,针对鉴定出的精胺合成酶基因(MAA_02088,Mrsps),利用农杆菌介导的同源重组方法获得Mrsps敲除株ΔMrsps。与野生型相比,ΔMrsps营养生长和产孢能力下降,对氯化钠和紫外照射耐受性增强。大蜡螟幼虫毒力分析表明,浸渍和注射两种情况下ΔMrsps致病力降低,半致死时间(LT50:6.71和4.75 d)比野生型(LT50:5.17和4.19 d)显著增加。Mrsps敲除后不影响附着胞形成率和蝉翅穿透能力,但会显著下调昆虫血腔定殖相关基因的表达量。这些结果说明精胺合成酶Mr SPS参与调控罗伯茨绿僵菌的生长发育、外界胁迫应答和致病力。This study aimed at characterizing the function of spermine synthase gene(MAA_02088,Mrsps)by knocking it out with Agrobacterium-mediated homologous recombination in Metarhizium robertsii.Compared to the wild-type,knocking strainΔMrsps showed defects in radial growth and conidial production,and increase in tolerance to ultraviolet irradiation and NaCl.The results of bioassay showed reduction of virulence ofΔMrsps infecting Galleria mellonella under the conditions of topical inoculation and injection,and the LT50s of insect infected byΔMrsps were markedly longer than those of insect infected by the wild-type.Different fungal strains exhibited similar abilities on appressorium formation and cicada wing penetration,but the expression of the genes involved in hemocoel colonization were significantly downregulated after Mrsps deletion.These results indicate that spermine synthase is involved in the regulation of development,stress response and pathogenicity of M.robertsii.

关 键 词：绿僵菌 精胺合成酶 生长发育 胁迫应答 致病力 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]