全血标本中分离出阿尔塔米拉金色单胞菌及其全基因组分析  

Isolation of Aureimonas altamirensis from whole blood and whole genome sequence analysis

作　　者：田丽梅[1] 金丹婷[2] 茆海丰[2] Tian Limei;Jin Danting;Mao Haifeng(Department of Infection Management,the First People′s Hospital of Lianyungang,Lianyungang 222000,China;Department of Clinical Laboratory,the First People′s Hospital of Lianyungang,Lianyungang 222000,China)

机构地区：[1]连云港市第一人民医院感染管理科,连云港222000 [2]连云港市第一人民医院检验科,连云港222000

出　　处：《中华微生物学和免疫学杂志》2022年第10期777-783,共7页Chinese Journal of Microbiology and Immunology

摘　　要：目的了解一株分离自临床血液标本中罕见菌——阿尔塔米拉金色单胞菌(Aureimonas altamirensis)的生物学特点、鉴定方法、基因组结构和临床意义。方法对一株血培养分离菌的培养特性、简单生化特征观察了解;用实验室常规的生化鉴定仪、MALDI-TOF质谱仪和16S rRNA基因序列分析鉴定分离菌,并将测得的16S rRNA基因序列与相关菌株构建16S rRNA系统进化树;对分离菌基因组进行测序和组装,利用相关软件对基因组作基因预测和功能注释。将分离菌与GenBank数据库中收录的相近菌株作基于31个House-Keeping基因进化树分析和全基因组同源核苷酸平均一致性(average nucleotide identity,ANI)分析。结果分离菌为过氧化氢酶、脲酶、氧化酶反应阳性的革兰阴性无芽孢需氧小杆菌,在血平板上生长缓慢,不能被自动化细菌生化鉴定仪和MALDI-TOF质谱鉴定仪可靠鉴定,16S rRNA基因序列分析和进化树显示:该菌16S rRNA基因序列与GenBank收录的NML070722和IARI-ABL-26阿尔塔米拉金色单胞菌16S rRNA基因序列(序列号为EU442518.1和KC581669.1)一致性最高,相似性均为99.93%。全基因测序结果表明该菌基因组(国家微生物科学数据中心基因序列号:NMDC60043566)总长为4332458 bp,GC含量65.14%;编码4088个基因,功能基因注释显示功能基因主要富集于蛋白质、氨基酸、碳水化合物转运和代谢类功能区,致病基因分析预测到两个可靠性高毒力因子基因,未预测到耐药基因。基因组House-Keeping基因进化树分析显示该菌与阿尔塔米拉金色单胞菌DSM21988和C2P003(NCBI基因组序列号为GCF 001463885.1和GCF 000800175.1)高度同源,但全基因组ANI分析显示其基因组与两菌存在较大差异。结论在国内临床标本中分离出罕见的阿尔塔米拉金色单胞菌,其生物学和基因组特点还没有被充分认识,目前常规方法难以正确鉴定,其对免疫低下患者的致病性和在临床标本中�Objective To understand the biological characteristics,identification methods,genome structure and clinical significance of a rare strain of Aureimonas altamirensis isolated from clinical blood sample.Methods The culture and biochemical characteristics of a strain isolated from blood culture were observed.The routine biochemical identification methods,MALDI-TOF mass spectrometer and 16S rRNA gene sequencing were used to identify the isolate.A phylogenetic tree based on the 16S rRNA gene sequences of the isolate and related strains was constructed.The genome of the isolated strain was sequenced and assembled,and gene prediction and functional annotation were made using related software.Phylogenetic analysis based on 31 house-keeping genes and genome-wide average nucleotide identity(ANI)analysis were conducted between the isolate and other Aureimonas sp.strains.Results The isolated bacteria were gram-negative bacillus positive for catalase,urease and oxidase.It grew slowly on blood plate and could not be reliably identified by automatic bacterial biochemical identification systems or MALDI-TOF mass spectrometry.Results of the 16S rRNA gene sequencing and phylogenetic tree showed that the 16S rRNA gene sequence of the isolated strain was highly homologous to the strains of Aureimonas altamirensis NML070722 and IARI-ABL-26(GenBank accession number:EU442518.1 and KC581669.1)in GenBank,and the gene sequence similarity was 99.93%.The total genome(National Microbiology Data Center genome accession number:NMDC60043566)length was 4332458 bp and GC content was 65.14%.There were 4088 protein-coding genes and functional gene annotation showed that functional genes were mainly enriched in protein,amino acid,carbohydrate transport and metabolic functional regions.Pathogenic gene analysis predicted two high reliable virulence factor genes,but no drug resistance genes.House-keeping gene phylogenetic tree analysis showed that this strain was highly homologous to Aureimonas altamirensis strains of DSM21988 and C2P003(GenBank access

关 键 词：阿尔塔米拉金色单胞菌 16S rRNA基因序列分析 全基因组测序 

分 类 号：R446.5[医药卫生—诊断学]