阻塞性睡眠呼吸暂停低通气综合征的蛋白质组学研究  

Proteomic study of obstructive sleep apnea hypopnea syndrome

作　　者：刘岩[1] 于丹[1] 张慧慧[1] 郭海仙[1] 刘悦[1] 张悦[1] 聂帅 靳凤颖 文连姬[1] LIU Yan;YU Dan;ZHANG Huihui;GUO Haixian;LIU Yue;ZHANG Yue;NIE Shuai;JIN Fengying;WEN Lianji(Department of OtolaryngologyHead and Neck Surgery,the Second Hospital of Jilin University,Changchun,Jilin,130041,China)

机构地区：[1]吉林大学第二医院耳鼻咽喉头颈外科,吉林长春130041

出　　处：《中国耳鼻咽喉头颈外科》2022年第9期568-572,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：吉林省自然科学基金(20200201455JC);吉林省自然科学基金(20210101301JC);吉林省卫生专项项目(2020SCZT049)。

摘　　要：目的 通过血清蛋白质组学方法分析阻塞性睡眠呼吸暂停低通气综合征(OSAHS)的蛋白质表达差异,并分析差异蛋白对临床诊断的价值。方法 纳入10例OSAHS患者和10例健康受试者作为训练组,对外周血清进行蛋白质组学研究,筛选出有差异表达显著的蛋白,并进行生物信息学分析;继续纳入33例OSAHS患者和25名健康受试者作为验证组,检测外周血清中差异蛋白的表达水平,并结合已有文献,分析有意义的差异蛋白对临床诊断的价值。结果 与健康受试者相比,OSAHS患者血清表达上调蛋白32个,表达下调蛋白44个。与健康受试者相比,OSAHS患者血清差异表达蛋白主要与免疫功能、细胞分泌和金属离子结合等相关。KEGG通路富集结果显示,差异蛋白主要富集在唾液分泌(salivary secretion)和胃酸分泌(gastric acid secretion)通路中。ROC曲线分析结果显示,超氧化物歧化酶(SOD)预测OSAHS的曲线下面积(AUC)为0.878(95%CI=0.788~0.967),敏感度为0.840,特异度为0.758;过氧化氢酶(CAT)预测OSAHS的AUC为0.845(95%CI=0.731~0.959),敏感度为0.840,特异度为0.788。结论 OSAHS患者与健康受试者血清差异表达蛋白主要与与免疫功能、细胞分泌和金属离子结合等相关。OSAHS患者血清SOD和CAT表达均显著下降,SOD和CAT对OSAHS均具有一定的诊断效能。OBJECTIVE To analyze the protein expression difference of obstructive sleep apnea hypopnea syndrome(OSAHS) by serum proteomics method,and to analyze the value of differential protein in clinical diagnosis.METHODS Ten patients with OSAHS and 10 healthy subjects were selected as the training group.The proteomics of peripheral serum was studied,and the proteins with significant differential expression were screened and analyzed by bioinformatics;33 patients with OSAHS and 25 healthy subjects were included as the validation group.The expression levels of differential proteins in peripheral serum were detected.Combined with the existing literature,the value of significant differential proteins in clinical diagnosis was analyzed.RESULTS Compared with healthy subjects,32 proteins were up-regulated and 44 proteins were down regulated in OSAHS patients.Compared with healthy subjects,the differentially expressed proteins in serum of OSAHS patients were mainly related to immune function,cell secretion and metal ion binding.KEGG pathway enrichment results showed that the differential proteins were mainly enriched in salivary secretion and gastric acid secretion pathways.The area under the curve(AUC) of SOD was 0.878 (95%CI:0.788-0.967),the sensitivity was 0.840,and the specificity was 0.758;The AUC of CAT was 0.845(95%CI:0.731-0.959),the sensitivity was 0.840,and the specificity was 0.788.CONCLUSION The differentially expressed proteins in serum of OSAHS patients and healthy subjects were mainly related to immune function,cell secretion and metal ion binding.The expression of SOD and CAT in serum of OSAHS patients were significantly decreased,and the diagnosis of OSAHS with SOD and CAT was good.

关 键 词：睡眠呼吸暂停 阻塞性 蛋白组学 超氧化物歧化酶 过氧化氢酶 诊断 

分 类 号：R766[医药卫生—耳鼻咽喉科]