单细胞转录组测序技术及其在植物中的应用  

Single Cell Transcriptome Sequencing Technology and Its Application in Plants

作　　者：张舒婷[1] 张雪莹 朱晨 李卓蕴 傅卓然 张梓浩[1] 赖钟雄[1] 林玉玲[1] ZHANG Shuting;ZHANG Xueying;ZHU Chen;LI Zhuoyun;FU Zhuoran;ZHANG Zihao;LAI Zhongxiong;LIN Yuling(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《园艺学报》2022年第10期2163-2173,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31672127);福建省自然科学基金项目(2020J01543);福建省高原学科建设经费项目(102/71201801101);福建农林大学创新基金项目(CXZX2019033S,CXZX2018078)。

摘　　要：近年来发展起来的基于高通量测序技术的高分辨率单细胞转录组测序技术,能够在单细胞水平解析植物组织的异质性,鉴定组织中的不同细胞类型,构建不同细胞类型的连续分化轨迹及其转录调控网络,并能够寻找组织中的稀有细胞类型,深入解析组织发育的分子机制。为探究单细胞转录组测序技术在植物中的应用,本文中对单细胞转录组测序技术的发展及其在植物中的应用进行了综述。首先,介绍了单细胞转录组测序技术的发展进程;其次,分析其在鉴定植物组织不同细胞类型、筛选不同细胞类型特异表达标记基因及解析植物组织发育过程的连续分化轨迹及其转录调控网络的应用中所取得的进展;最后,总结单细胞转录组测序技术在植物应用中存在的挑战及未来的研究方向。以期为单细胞转录组测序技术在更多植物物种上得以应用提供参考。High-resolution single-cell RNA sequencing(scRNA-seq)technology based on highthroughput sequencing technology has been developed recently to resolve the heterogeneity of plant tissues at the single-cell level,identify distinct cell types in single tissue,and construct continuous cell differentiation trajectories and the transcriptional regulatory networks,in addition,it can search for rare cell types in tissues,thus deeply analyze molecular mechanisms of tissue development.To explore the application of scRNA-seq technology in plants,this paper reviews the progress of scRNA-seq technology and its application in plants.Firstly,the development course of scRNA-seq technology was overviewed;subsequently,we analyzed the progress of scRNA-seq technology in the identification of different plant tissue cell types,screening of specific expression marker genes for different cell types,and analyzing the continuous differentiation trajectories and transcriptional regulatory networks during the development of different plant tissues;finally,we summarized the challenges and future research directions of the scRNA-seq technology applicating in plants,and aims to provide reference for the application of scRNA-seq technology in more plant species in the future.

关 键 词：单细胞RNA测序 植物组织 细胞异质性 标记基因 拟时分析 

分 类 号：Q813[生物学—生物工程]