Microbe-derived non-necrotic glycoside hydrolase family 12 proteins act as immunogenic signatures triggering plant defenses  

作　　者：Lan Wang Hanmei Liu Mingmei Zhang Yu Ye Lei Wang Jinyi Zhu Zhaodan Chen Xiaobo Zheng Yan Wang Yuanchao Wang 

机构地区：[1]Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China [2]Key Laboratory of Soybean Disease and Pest Control(Ministry of Agriculture and Rural Affairs),Nanjing Agricultural University,210095,Nanjing,China [3]The Key Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Integrative Plant Biology》2022年第10期1966-1978,共13页植物学报（英文版）

基　　金：supported by grants from the Natural Science Funds for Distinguished Young Scholars of Jiangsu Province (BK20190027);from the China National Funds (32172423, 31872927 and 31721004);by China Agriculture Research System (CARS-004-PS14);by “the Fundamental Research Funds for the Central Universities” (KJJQ202002 and JCQY201904)

摘　　要：Plant pattern recognition receptors(PRRs)are sentinels at the cell surface sensing microbial invasion and activating innate immune responses.During infection,certain microbial apoplastic effectors can be recognized by plant PRRs,culminating in immune responses accompanied by cell death.However,the intricated relationships between the activation of immune responses and cell death are unclear.Here,we studied the glycoside hydrolase family12(GH12)protein,Ps109281,secreted by Phytophthora sojae into the plant apoplast during infection.Ps109281 exhibits xyloglucanase activity,and promotes P.sojae infection in a manner dependent on the enzyme activity.Ps109281 is recognized by the membranelocalized receptor-like protein RXEG1 and triggers immune responses in various plant species.Unlike other characterized GH12 members,Ps109281 fails to trigger cell death in plants.The loss of cell death induction activity is closely linked to a sequence polymorphism at the Nterminus.This sequence polymorphism does not affect the in planta interaction of Ps109281 with the recognition receptor RXEG1,indicating that cell death and immune response activation are determined using different regions of the GH12 proteins.Such GH12 protein also exists in other Phytophthora and fungal pathogens.Taken together,these results unravel the evolution of effector sequences underpinning different immune outputs.

关 键 词：apoplastic effector cell death glycoside hydrolases immune recognition immune responses 

分 类 号：S432.2[农业科学—植物病理学]