24种中药成分对海战伤病原菌的抑制作用研究  

Research on active antibacterial ingredients of 24 bio-active traditional Chinese medicine against pathogenic bacteria in naval battle injuries

作　　者：胡波 孔君南 翟祥宁 刘小宇 于豪冰 刘李娜 Hu Bo;Kong Junnan;Zhai Xiangning;Liu Xiaoyu;Yu Haobing;Liu Lina(Research Department of Naval Medical Center,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学海军特色医学中心研究部,上海200433 [2]海军军医大学基础医学院学员一队

出　　处：《海军医学杂志》2022年第10期1094-1097,共4页Journal of Navy Medicine

基　　金：海军军医大学大学生创新孵化项目(FH2019021)。

摘　　要：目的 筛选24种中药单体中有效抑制海洋弧菌的成分。方法 采用纸片法对中药单体进行初筛,采用微量肉汤稀释法对初筛阳性的成分进行复筛和抗菌谱研究。结果 石吊兰素、芹菜素、盐酸白屈菜红碱和雷公藤红素对创伤弧菌(Vibrio vulnificus,VV)和副溶血弧菌(Vibrio parahaemolyticus,VP)有不同程度的抑制作用,雷公藤红素抑菌作用最强,其抑制VV和VP的最低抑菌浓度(minimum inhibitory concentration,MIC)分别为2.2μmol/L和1.1μmol/L;其次为盐酸白屈菜红碱,其抑制VV和VP的MIC均为2.6μmol/L。这2种化合物对粪肠球菌均具有很强的抑制作用,雷公藤红素和盐酸白屈菜红碱的MIC分别为4.4μmol/L和10.4μmol/L。盐酸白屈菜红碱还对金黄色葡萄球菌有抑制作用,MIC为41.6μmol/L。24种中药单体对溶藻弧菌(Vibrio alginolyticus,VA)均无抑制作用。结论 雷公藤红素和盐酸白屈菜红碱能够特异性抑制海洋弧菌的成分,为治疗海洋弧菌类感染提供了研究基础。Objective To screen and obtain active antibacterial ingredients against marine vibrio from 24 bio-active traditional Chinese medicine simplex.Methods Disc diffusion test was used to screen antibacterial ingredients from traditional Chinese medicine ingredients against 3 vibrio strains.The minimum inhibition concentration and antibacterial spectrum of antimicrobial activities of the ingredients were evaluated by broth dilution method.Results Lysionotin,apigenin,tripterine and chelerythrine displayed various antibacterial activities against Vibrio vulnificus(VV) and Vibrio parahaemolyticus(VP),with tripterine having strongest antimicrobial activity.The minimum inhibitory concentrations(MIC) against VV and VP were respectively2.2 μmol/L and 1.1 μmol/L.Next came chelerythrine with the MIC against VV and VP having the same value of 2.6 μmol/L.In addition,these two ingredients displayed very strong inhibition against the growth of Enterococcus Faecalis with the MIC values of 4.4 pμmol/L and 10.4 μmol/L.Chelerythrine could inhibit the growth of Staphylococcus aureus with the MIC value of 41.6 μmol/L.However,the 24 traditional Chinese medicine simplexes did not display inhibition against the growth of Vibrio alginolyticus(VA).Conclusion Tripterine and chelerythrine could specifically inhibit marine vibrio,and our research results would provide drug reserve against marine vibrio infection.

关 键 词：海洋弧菌 雷公藤红素 白屈菜红碱 抑菌作用 中药 

分 类 号：R285[医药卫生—中药学]