一种灭活型样本保存液在新型冠状病毒核酸快速检测中的应用评价  

Evaluation of an inactivated sample preservation solution for rapid detection of SARS-CoV-2

作　　者：赵莉[1] 陆柔剑[1] 叶飞[1] 黄保英[1] 王慧娟[1] 谭文杰[1] Zhao Li;Lu Roujian;Ye Fei;Huang Baoying;Wang Huijuan;Tan Wenjie(National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华临床实验室管理电子杂志》2022年第1期17-22,共6页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：国家重点研发计划(2021YFC0863300、2020YFC0840900)。

摘　　要：目的评价含表面活性剂Triton X-100及NP40的灭活型样本保存液对新型冠状病毒(SARS-CoV-2)的灭活效果及其对核酸快速检测的影响。方法通过测定灭活型样本保存液对SARS-CoV-2灭活前后病毒滴度变化,评价保存液的灭活效果;用灭活型样本保存液洗脱含有SARSCoV-2模拟病毒的咽拭子样本,评价样本保存液对SARS-CoV-2核酸快速检测最低检测限和精密度的影响,并将SARS-CoV-2在灭活型样本保存液中保存不同时间以评价保存液对SARS-CoV-2核酸检测的影响。结果灭活型样本保存液在5 min内可使SARS-CoV-2滴度至少下降2.76×105倍;灭活型样本保存液对SARS-CoV-2核酸检测的灵敏度无影响,对SARS-CoV-2核酸检测Ct值的CV小于5%;SARS-CoV-2在灭活型样本保存液中于25℃或2℃~8℃条件下可至少稳定保存4 d。结论含表面活性剂Triton X-100及NP40的灭活型样本保存液可有效灭活SARS-CoV-2病毒,且灭活后病毒核酸检测不受影响,适用于核酸快速检测。Objective To evaluate the inactivation effect of sample preservation solution containing Triton X-100 and NP40 on novel coronavirus SARS-CoV-2 and its effect on rapid nucleic acid detection.Methods The virus inactivation effect was assessed by measuring the change of SARS-CoV-2 virus titer before and after the treatment with sample preservation solution.The influence on the minimum detection limit and precision of SARS-CoV-2 nucleic acid detection were evaluated by elution of throat swab samples containing SARS-CoV-2 simulated virus.The stability of nucleic acid of SARS-CoV-2 in the sample preservation solution was evaluated by spiking the SARS-CoV-2 virus in the solution and storing for different time.Results The SARS-CoV-2 virus titer decreased at least 2.76×105 times after being treated by the sample preservation solution in 5 minutes.The sample preservation solution had no effect on the sensitivity of SARS-CoV-2 nucleic acid detection,and the CV of SARS-CoV-2 nucleic acid detection Ct value was less than 5%.The SARS-CoV-2 virus can be stored in the sample preservation solution for at least 4 days at 25℃or 2~8℃.Conclusion The sample storage solution containing surfactants Triton X-100 and NP40 can effectively inactivate virus,and the virus nucleic acid detection is not affected after inactivation,which is suitable for rapid nucleic acid detection.

关 键 词：冠状病毒科 器官保存液 表面活性剂 核酸类 

分 类 号：R563.1[医药卫生—呼吸系统]