小肠电刺激减少肥胖大鼠进食时间  

The eating duration was reduced by intestinal electrical stimulation in obese rats

作　　者：叶晓玲 张宇[1,3] 万新月 Ye Xiaoling;Zhang Yu;Wan Xinyue(Department of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Ultrasound imaging,The First Affiliated Hospital of Zhejiang University Medical College,Hangzhou 310000,China;Department of Gastroenterology,Henan Provincial People’s Hospital,People’s Hospital of Zhengzhou University,Zhengzhou 450003,China)

机构地区：[1]武汉大学人民医院消化内科,武汉430060 [2]浙江大学医学院附属第一医院超声影像科,杭州310000 [3]河南省人民医院消化内科,郑州450003

出　　处：《中华肥胖与代谢病电子杂志》2022年第2期93-96,共4页Chinese Journal Of Obesity and Metabolic Diseases：Electronic Edition

基　　金：国家自然科学基金青年项目(81800481)。

摘　　要：目的通过观察小肠电刺激(IES)对肥胖大鼠进食模式的影响,初步探索小肠电刺激的减重机制。方法16只饮食诱导肥胖大鼠(DIO)被纳入试验。使用BioDAQ系统记录4组不同刺激时长(IES-0h,IES-1h,IES-6h和IES-12h)下DIO大鼠的进食模式。使用酶联免疫吸附法(ELISA)检测DIO大鼠餐后血清胰高血糖素样肽-1(GLP-1)及瘦素(Leptin)的表达水平。结果4组日均摄食量分别为(22.3±2.9)g、(21.9±3.7)g、(19.6±4.3)g和(17.5±5.1)g,呈现刺激时长依赖性下降趋势;大鼠日均进食次数无明显差异,但日均进食时间明显缩短。IES-12h组为(112.8±50.3)min对比对照组IES-0h组(147.9±52.1)min明显缩短(P<0.01)。DIO大鼠接受IES刺激1 h后餐后血清GLP-1对比对照组明显升高,而两组瘦素表达水平无差异。结论小肠电刺激通过减少DIO大鼠进食时间来减重,而GLP-1可能在其中发挥了作用。Objective Investigating the mechanism of intestinal electrical stimulation(IES)induced weight loss in obese rats by observing the changes of meal pattern.Methods Sixteen diet-induced obese rats(DIO)were used in the experiment.The BioDAQ system was used to record events of food intake.And the changes of meal pattern of obese rats with different stimulation duration(0 h,1 h,6 h and 12 h)were compared.Lastly,the levels of serum glucagon-like peptide-1(GLP-1)and Leptin were tested by enzyme-linked immunosorbent assay(ELISA).Results The average daily food intake with four different stimulation duration were(22.3±2.9)g,(21.9±3.7)g,(19.6±4.3)g and(17.5±5.1)g,respectively.This was a stimulation time-dependent downward trend.There was no significant difference in the average daily feeding times of rats,but the eating duration were significantly shortened,and the average eating duration of 12hours stimulation(IES-12h)was significantly shorter than that of IES-0h,(112.8±50.3)min vs.(147.9±52.1)min.When DIO rats received one hour stimulation,the level of postprandial serum GLP-1 was significantly higher than that of sham-IES,but there was no difference on the level of Leptin between the two groups.Conclusions IES caused the weight loss of obese rats by reducing the eating duration,and GLP-1 may play a role in it.

关 键 词：小肠电刺激 肥胖 胰高血糖素样肽-1 机制 

分 类 号：R589.2[医药卫生—内分泌]