1株海洋真菌Aspergillus sp.WJP1次级代谢产物及其活性研究  

Research on secondary metabolites and their activities from a marine fungus Aspergillus sp. WJP1

作　　者：逯佩玉 贾佳 毕洪凯 梁俊玉[1] 刘玲[2] LU Pei-yu;JIA Jia;BI Hong-kai;LIANG Jun-yu;LIU Ling(College of Life Sciences,Northwest Normal University,Lanzhou 730070,China;State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;School of Basic Medical Sciences,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [3]南京医科大学基础医学院,江苏南京211166

出　　处：《中国海洋药物》2022年第5期13-20,共8页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(32022002)资助。

摘　　要：目的 研究1株海洋来源的真菌Aspergillus sp. WJP1的次级代谢产物及其生物活性。方法 利用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备高效液相色谱等方法,对该菌株发酵提取物进行分离和纯化;通过核磁共振波谱(NMR)和质谱(MS)等方法并结合相关文献数据比对,鉴定化合物的结构;采用肉汤微量稀释法评价化合物的抗菌活性。结果 从该菌株中分离鉴定了10个化合物,分别是(S)-(+)-11-dehydrosydonic acid(1)、sydonic acid(2)、hydroxysydonic acid(3)、(+)-austrosene(4)、(7S,11S)-(+)-12-hydroxysydonic acid(5)、(7S,11S)-(+)-11,12-dihydroxysydonic acid(6)、7-O-methylsydonic acid(7)、aspergillusene B(8)、yicathin C(9)和emodin(10);体外抗菌结果显示,化合物9和10对幽门螺杆菌及金黄色葡萄球菌均有良好的抑制作用。Objective To investigate the secondary metabolites and their activities of a marine-derived fungus Aspergillus sp. WJP1. Methods The silica gel column chromatography, SephadexLH-20 gel column chromatography and high-performance liquid chromatography(HPLC) were used to isolate and purify the secondary metabolites from the fermented extracts of the fungus Aspergillus sp. WJP1. Their structures were elucidated on the basis of NMR and MS spectroscopic data and by comparison with the data of literature. Their antibacterial activities were evaluated by microdilution method. Results Ten compounds were isolated and identified as(S)-(+)-11-dehydrosydonic acid(1), sydonic acid(2), hydroxysydonic acid(3),(+)-austrosene(4),(7S,11S)-(+)-12-hydroxysydonic acid(5),(7S,11S)-(+)-11,12-dihydroxysydonic acid(6), 7-O-methylsydonic acid(7), aspergillusene B(8), yicathin C(9) and emodin(10). Compounds 9 and 10 showed antibacterial activities against Helicobacter pylori and Staphylococcus aureus in vitro.

关 键 词：曲霉属 次级代谢产物 抗菌活性 

分 类 号：R931.77[医药卫生—生药学]