同型半胱氨酸上调miR-488-3p表达诱导MPC-5小鼠肾小球足细胞凋亡  

Homocysteine induces glomerular podocyte apoptosis via up-regulation of miR-488-3p expression in MPC-5 mice

作　　者：揭育祯 丁宁[1,2] 谢琳 刘昆[1,2,3] 梁昪平 马飞 马胜超 姜怡邓 卢冠军[2,3] JIE Yuzhen;DING Ning;XIE Lin;LIU Kun;LIANG Bianping;MA Fei;MA Shengchao;JIANG Yideng;LU Guanjun(Ningxia Medical University;Key Laboratory of Metabolic Cardiovascular Diseases,National Health Commission;Department of Urology,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学 [2]国家卫生健康委代谢性心血管疾病研究重点实验室 [3]宁夏医科大学总医院泌尿外科,宁夏银川750004

出　　处：《细胞与分子免疫学杂志》2022年第9期801-806,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金地区项目(82060139,81760139);国家自然科学基金青年项目(81900273);宁夏回族自治区重点研发计划重点项(2018BEG02004)。

摘　　要：目的探讨微小RNA-488-3p(miR-488-3p)在同型半胱氨酸(Hcy)诱导足细胞凋亡中的作用。方法体外培养足细胞分别采用0μmol/L Hcy(对照组)和80μmol/L Hcy(Hcy组)处理48 h,流式细胞术检测足细胞凋亡率,Western blot法检测B细胞淋巴瘤因子2(Bcl2)、Bcl2相关X蛋白(BAX)和胱天蛋白酶3(caspase-3)的蛋白表达,实时荧光定量PCR检测miR-488-3p的表达;用miR-488-3p抑制物(miR-488-3p inhibitor)转染足细胞,检测转染效率和足细胞凋亡水平。结果与对照组相比,Hcy组中足细胞凋亡率、BAX和caspase-3表达水平显著升高,而Bcl2表达则显著降低;且Hcy显著促进足细胞中miR-488-3p的表达。转染miR-488-3p inhibitor后,足细胞凋亡率、BAX及caspase-3蛋白表达明显降低,而Bcl2表达显著升高。结论Hcy通过上调miR-488-3p表达而促进足细胞凋亡。Objective To explore the role of microRNA-488-3p(miR-488-3p)in podocytes apoptosis induced by homocysteine(Hcy).Methods Flow cytometry was employed to analyze the ratio of podocytes apoptosis after treated with 0μmol/L Hcy(control group)or 80μmol/L Hcy(Hcy group)for 48 hours.The expression levels of B-cell lymphoma 2(Bcl2),Bcl2-related X protein(BAX),and caspase-3 were measured by Western blot analysis in podocytes,after cells were treated with 80μmol/L Hcy(Hcy group)for 48 hours,and the expression of miR-488-3p was detected by real-time PCR.The transfection and apoptosis ratio of podocytes were also detected after cells were transfected with miR-488-3p inhibitor.Results The apoptosis rate of podocytes increased in cells treated with Hcy,compared with control group.The expression levels of BAX and caspase-3 increased significantly in Hcy group,while Bcl2 expression was suppressed by Hcy.Furthermore,the expression of miR-488-3p increased in Hcy-induced podocyte.On the contrary,podocyte showed an decreased apoptosis rate,expression levels of BAX and caspase-3 decreased after cells were transfected with miR-488-3p inhibitor.However,Bcl2,which was not in this line,showed an increase when the cells transfected with miR-488-3p inhibitor.Conclusion Hcy promotes apoptosis of podocyte by up-regulating the expression of miR-488-3p.

关 键 词：同型半胱氨酸(Hcy) 足细胞 微小RNA-488-3p(miR-488-3p) 细胞凋亡 

分 类 号：R965[医药卫生—药理学] Q24[医药卫生—药学] R392.12[生物学—细胞生物学] R692.6R392-33