视黄醇脱氢酶和视网膜G蛋白偶联受体在形觉剥夺性近视眼小鼠中表达特征的实验研究  

Retinol dehydrogenase and rpe-retinal G protein-coupled receptor expression patterns in mice after monocular deprivation

作　　者：刘添添 陈冰洁[1] 刘陇黔[1] Liu Tiantian;Chen Bingjie;Liu Longqian(Department of Ophthalmology,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院眼科,成都610041

出　　处：《中华眼科医学杂志（电子版）》2022年第1期6-11,共6页Chinese Journal of Ophthalmologic Medicine(Electronic Edition)

基　　金：四川省科技计划重点研发项目(21ZDYF1415);美国中华医学基金会应用技术项目(0040205502017)。

摘　　要：目的探讨视黄醇脱氢酶(RDH)和视网膜G蛋白偶联受体(RGR)在形觉剥夺性近视眼小鼠中的表达特征。方法采用数字表法将4周龄C57BL6/J小鼠100只(200只眼)随机分为对照组和近视组,每组各50只(100只眼)。采用自制的遮光眼罩形觉剥夺小鼠的单眼视力,构建近视眼动物模型。右眼形觉剥夺4周后,采用红外偏心摄影验光仪测量小鼠的屈光度。采用手持数字显微镜测量小鼠的眼轴长度。采用实时逆转录聚合酶链反应测量小鼠全视网膜组织中Rdh5、Rdh10及Rgr基因信使核糖核酸(mRNA)的相对表达量。采用蛋白质免疫印迹法测量小鼠全视网膜组织中RDH5、RDH10及RGR蛋白的表达水平。两组小鼠屈光度、眼轴长度、基因的mRNA相对表达量及蛋白质相对灰度值采用±s表示。两组小鼠屈光度的比较采用配对t检验;眼轴长度、基因mRNA相对表达量及蛋白质相对灰度值的总体差异采用单因素方差分析,当差异有统计学意义时,再进一步采用LSD检验两两比较。结果近视组小鼠遮盖眼和对侧眼的屈光度分别为(-5.631±4.052)D和(4.231±2.828)D。近视组小鼠遮盖眼呈现出明显的近视漂移,两者比较的差异有统计学意义(t=-10.91,P<0.05)。对照组右眼和左眼的屈光度分别为(3.774±4.079)D和(4.171±4.425)D,两者比较的差异无统计学意义(t=-1.344,P>0.05)。近视组小鼠遮盖眼、对侧眼及对照组眼轴分别为(3305.0±86.4)μm、(3221.0±90.0)μm及(3232.0±68.6)μm。遮盖眼眼轴最长,三者比较的差异均有统计学意义(F=6.248,P<0.05)。近视组小鼠遮盖眼Rdh5、Rdh10及Rgr基因mRNA的相对表达量分别为(2.032±0.162)、(2.611±0.258)及(3.876±0.576),均略低于对侧眼。近视组小鼠遮盖眼Rdh5、Rdh10及Rgr基因的mRNA相对表达量高于对照组。经单因素方差分析,两组比较的差异均有统计学意义(F=38.671,88.510,78.557;P<0.05)。近视组和对照组小鼠RDH5、RDH10及RGR蛋白质相对灰度值的Objective The aim of this study was to examine the expression pattern differences of retinol dehydrogenase(RDH)and rpe-retinal G protein-coupled receptor(RGR)in deprivation myopic mice.Methods 100 four-week-old C57BL6/J mice(200 eyes)were randomly distributed into myopia group and control group,50 for each group(100 eyes).Monocular deprivation of was induced by covering the right eyes with handmade,translucent occluders,while the left eyes served as contralateral eyes.Mean-while,the control group received no treatment.After deprivation for four weeks,refraction errors were measured via an eccentric infrared photorefractor,and axial lengths were estimated using a hand-held digital microscope.The messenger ribonucleic acid(mRNA)transcript levels of Rdh5,Rdh10 and Rgr genes were detected using real-time reverse transcription polymerase chain reaction.The corresponding protein concentrations of these genes were detected by Western blot analysis.Refractive errors,axial length,mRNA levels and the relative gray value of protein were described as±s.The refraction interocular difference was used to compare by paired t-test.One-way ANOVA was used to determine statistic differences in axial length,mRNA levels and relative gray value of protein among multiple samples,and the LSD test was used for further pairwise comparisons.Results The refractive error of the covered myopic eyes and contralateral eyes in myopia group were(-5.631±4.052)D,(4.231±2.828)D,resepctively.There was a significant difference between them(t=-10.914,P<0.05).The control group was found to have refractive error for right and left eyes,which were(3.774±4.079)D and(4.171±4.425)D,respectively.There was not a significant difference between them(t=-1.344,P>0.05).The axial length of covered myopic eyes,contralateral eyes and control group were(3305.0±86.4)μm,(3221.0±90.0)μm and(3232.0±68.6)μm,respectively.After one-way ANOVA,there was a statistically significant difference among them(F=6.248,P<0.05).In the retinas of covered myopic eyes,the relative

关 键 词：形觉剥夺近视 视黄醇脱氢酶5 视黄醇脱氢酶10 视网膜G蛋白偶联受体 视网膜 

分 类 号：R285[医药卫生—中药学]