HPLC-波长切换法同时测定活血散中3个主要成分的含量  

Simultaneous Determination of Three Main Components in HuoX ue Powder by HPLC Wavelength Switching Method

作　　者：游旭彪 姚泽炳 YOU Xu-biao;YAO Ze-bing(Fuzhou Technical Center for Production License Examination of Industrial Products(Fuzhou Food and Drug Evaluation Center),Fuzhou 350011,China;Fuzhou Institute for Food and Drug Control,Fuzhou 350007,China)

机构地区：[1]福州市工业产品生产许可证审查技术中心(福州市食品药品审评中心),福建福州350011 [2]福州市食品药品检验所,福建福州350007

出　　处：《海峡药学》2022年第10期37-40,共4页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法同时测定活血散中栀子苷、芍药苷、柚皮苷的含量。方法采用HPLC-PDA法,色谱柱为Waters XBridge C_(18)柱(5μm,250 mm×4.6 mm),以乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速为1.0 m L·min^(-1);柱温为35℃;PDA检测器,对3个主要成分设定不同波长进行定量,其中芍药苷为230 nm,栀子苷为238 nm,柚皮苷为283 nm。结果栀子苷、芍药苷、柚皮苷分别在16.03~160.3μg·m L^(-1),10.02~100.2μg·m L^(-1),4.010~40.10μg·m L^(-1)内线性关系良好;平均回收率分别为98.8%,98.2%和98.1%;RSD分别为0.80%,1.23%和0.93%。结论本方法快速、简便、准确、重现性好,可作为控制活血散质量的方法。OBJECTIVE HPLC method was established for the simultaneous determination of gardenoside,paeoniflorin and naringin in HuoXue powder.METHODS HPLC-PDA method is adopted,and the chromatographic column is waters XBridge C_(18)column (5μm,250 mm×4.6 mm),gradient elution with acetonitrile-0.1%phosphoric acid solution as the mobile phase,and the flow rate is 1.0 m L·min^(-1);The column temperature is 35℃;PDA detector,set different wavelengths to quantify the three main components,including paeoniflorin 230 nm,gardenoside238 nm,naringin 283 nm.RESULTS The linear relationship of gardenoside,paeoniflorin and naringin were good in the range of 16.03-160.3μg·m L^(-1),10.02-100.2μg·m L^(-1),4.010-40.10μg·m L^(-1)respectively,The average recoveries were 98.8%,98.2%and 98.1%respectively;RSD was 0.80%,1.23%and 0.93%respectively.CONCLUSION The method is rapid,simple,accurate and reproducible,and can be used to control the quality of HuoXue Power.

关 键 词：活血散 栀子苷 芍药苷 柚皮苷 波长切换 多成分测定 质量控制 

分 类 号：R927[医药卫生—药学]