海水胁迫对两种乌桕叶片保护系统的影响  

Effects of Seawater Stress on Two Kinds of Sapium sebiferum(L.) Roxb.Leaf Protection Systems

作　　者：张焕仕 宰学明 陈洋 何河 马艳 隋德宗[2] Zhang Huanshi;Zai Xueming;Chen Yang;He He;Ma Yan;Sui Dezong(College of Horticulture,Jinling Institute of Technology,Nanjing 210038,China;Jiangsu Academy of Forestry,Nanjing 211153,China)

机构地区：[1]金陵科技学院园艺园林学院,江苏南京210038 [2]江苏省林业科学研究院,江苏南京211153

出　　处：《中国野生植物资源》2022年第11期29-34,38,共7页Chinese Wild Plant Resources

基　　金：江苏省林业科技创新与推广项目(LYKJ【2020】01)。

摘　　要：利用盆栽试验研究了不同浓度海水(CK,9%,15%)胁迫在不同处理时间(4 h、8 h、12 h、24 h)下对两年生海滨晚霞乌桕(晚霞)和海滨紫晶乌桕(紫晶)幼苗叶片保护系统的影响。结果表明,晚霞乌桕脯氨酸(Pro)含量随着处理时间延长先降低后升高,紫晶乌桕Pro积累量则随时间延长先升高后降低。晚霞乌桕超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均随时间延长先增加后降低,15%海水处理的过氧化氢酶(CAT)活性随着时间延长持续下降;海水处理抑制了紫晶乌桕SOD和POD活性,只有POD活性在处理24 h时显著高于对照。海水胁迫下晚霞乌桕丙二醛(MDA)含量随时间延长先降低后升高,紫晶乌桕MDA含量只有在15%海水处理24 h后显著高于其他处理。相关性分析表明,MDA含量与Pro含量、SOD活性呈显著负相关关系(P<0.05),而与POD活性则呈极显著负相关关系(P<0.01),而CAT活性与其他指标都不存在显著相关性(P>0.05)。本研究表明,晚霞乌桕在受到海水胁迫时主要通过提高POD活性清除ROS,紫晶乌桕则可能主要依靠提高CAT活性以抵御海水胁迫;相较晚霞乌桕,紫晶乌桕在受到高浓度海水长期胁迫时才会引起较严重的膜脂过氧化。A pot experiment was conducted to study the effects of different concentrations of seawater(CK,9 %,15 %)and different treatment time(4 h,8 h,12 h,24 h)on the leaf protection system of Haibin Wanxia(Wanxia)and Haibin Zijing(Zijing)Sapium sebiferum(L.)Roxb. seedlings. The results showed that the contents of proline(Pro)in Wanxia decreased first and then increased with the prolongation of treatment time,while the accumulation of Zijing Pro increased first and then decreased with the prolongation of treatment time. The activities of superoxide dismutase(SOD)and peroxidase(POD)increased first and then decreased with time. The activity of catalase(CAT)in 15% seawater treatment continued to decrease with time. Seawater treatment inhibited the activities of SOD and POD,only POD activity was significantly higher than that of the control at 24 h. Under seawater stress,contents of malondialdehyde(MDA)in Wanxia decreased first and then increased with time. And contents of MDA in Zijing was significantly higher than that in other treatments only after 15 % seawater treatment for 24 h. Correlation analysis showed that MDA content was significantly negatively correlated with Pro content and SOD activity(P < 0.05),while it was significantly negatively correlated with POD activity(P < 0.01),while CAT activity was not significantly correlated with other indicators(P > 0.05). This study showed that when Wanxia was exposed to seawater stress,it scavenged ROS mainly by increasing POD activity,while Zijing might mainly rely on increasing CAT activity to resist seawater stress. Compared with Wanxia,Zijing will cause more serious membrane lipid peroxidation when subjected to long-term stress of high concentration seawater.

关 键 词：海水胁迫 乌桕 耐盐性 保护系统 

分 类 号：Q945.78[生物学—植物学]