基于UPLC-MS/MS多反应监测的温心方人参皂苷Rb1、肉桂酸、芍药苷及小檗碱同时定性定量分析  

Content determination of ginsenoside Rb1,cinnamic acid,paeoniflorin and berberine in Wenxin Formula based on UPLC-MS/MS MRM mode

作　　者：王宗伟 李雪娜[1] 刘畅[1] 闫广利[1] 王喜军[1] 孙晖[1] Wang Zongwei;Li Xuena;Liu Chang;Yan Guangli;Wang Xijun;Sun Hui(Heilongjiang University of Chinese Medicine,Engineering Research Center of Efficiency Evaluation and Industrialization Development of Classical Prescriptions,Ministry of Education,Research Center of Chinmedomics,National Administration of TCM,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,教育部经典名方有效性评价及产业化开发工程研究中心,国家中医药管理局中医方证代谢组学研究中心,哈尔滨150040

出　　处：《国际中医中药杂志》2022年第10期1138-1143,共6页International Journal of Traditional Chinese Medicine

基　　金：黑龙江省应用技术研究与开发计划项目(GA18C004);黑龙江中医药大学科研基金项目(2018xy01)。

摘　　要：目的建立基于超高效液相色谱-串联质谱(UPLC-MS/MS)多反应监测(MRM)模式的温心方中人参皂苷Rb1、肉桂酸、芍药苷及小檗碱同时定性定量的分析方法,为温心方新药研发提供评价方法。方法采用Waters ACQUITY UPLCTM HSS T3(2.1 mm×100 mm,1.8μm)色谱柱,流动相为甲醇-0.03%甲酸水溶液,梯度洗脱,利用三重四极杆在MRM扫描模式下检测,以保留时间及相对丰度比(定性离子对/定量离子对)定性,以定量离子对峰面积进行定量研究。结果温心方中4种有效成分均被定性检出且线性关系良好;准确度、精密度、重复性、稳定性良好;回收率为96.24%~104.19%,RSD为1.29%~3.27%。结论该方法简便、准确、快速、灵敏,可用于温心方中4种有效成分的定性定量研究。Objective To establish a qualitative and quantitative analysis method of ginsenoside Rb1,cinnamic acid,paeoniflorin and berberine in Wenxin formula based on UPLC-MS/MS MRM model so as to provide a rapid and accurate evaluation method for the research and development of new drugs.Methods Adopting Waters ACQUITY UPLCTM HSS T3(2.1 mm×100 mm,1.8μm)and gradient elution was performed by mobile methanol-0.03% formic acid water.chromatographic column was used with electrospray ionization source in the scanning mode of multiple reactive ion monitoring(MRM)for ion separation and screening.The retention time and the relative abundance ratio(qualitative ion pairs/quantitative ion pairs)was used for qualitative analysis,while quantitative ion pairs was used for quantitative analysis.Results The four components tested in Wenxin Formula have been qualitatively detected and showed a good linear relationship,the method showed accuracy,precision,repeatability,and stability,the recovery rate was 96.24%-104.19% and RSD was 1.29%-3.30%.Conclusion The established method is simple,accurate,rapid and sensitive,which is reliable for the qualitative and quantitative study of the four main components in Wenxin Formula.

关 键 词：含量测定(中药) 温心方 超高效液相色谱-串联质谱 定性定量分析 质量控制 

分 类 号：R286.0[医药卫生—中药学]