基于UPLC-ESI-Q-TOF-MS/MS技术快速鉴定彝族药双参的化学成分  

Identification of Chemical Constituents in Triplostegia glandulifera Wall by UPLC-ESI-Q-TOF-MS/MS

作　　者：支雅婧 甄亚钦[1,2,3] 郗仲玟 田伟 王鑫国[1,2,3] 牛丽颖 ZHI Ya-jing;ZHEN Ya-qin;XI Zhong-wen;TIAN Wei;WANG Xin-guo;NIU Li-ying(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Hebei TCM Formula Granule Innovation Center,Shijiazhuang 050091,China;Hebei TCM Quality Evaluatio&Standardization Engineering Research Center,Shijiazhuang 050091,China)

机构地区：[1]河北中医学院,石家庄050091 [2]河北省中药配方颗粒技术创新中心,石家庄050091 [3]中药材品质评价与标准化河北省工程研究中心,石家庄050091

出　　处：《中国药学杂志》2022年第19期1611-1620,共10页Chinese Pharmaceutical Journal

基　　金：中央引导地方科技发展资金项目资助(206Z2501G);河北省重点研发计划项目资助(20372502D);河北省自然科学基金项目资助(H2019423050)。

摘　　要：目的应用超高效液相色谱-电喷雾/四极杆飞行时间串联质谱(UPLC-ESI-Q-TOF-MS/MS)技术快速鉴定双参药材的化学成分。方法采用Acquity UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm)分离,以体积分数0.1%甲酸水溶液(A相)-乙腈(B相)为流动相进行梯度洗脱,流速0.4 mL·min^(-1),采用电喷雾离子源(ESI),在正、负离子模式下采集数据,扫描范围m/z 100~2000,通过与对照品的保留时间及质谱数据信息对比,并结合精确相对分子质量、质谱裂解规律、质谱数据库(ChemicalBook,ChemSpider,Pubmed等)及相关文献,对双参的化学成分进行快速鉴定。结果从彝族药双参中共鉴定出59个化合物,其中包括11个环烯醚萜类、23个有机酸类、14个三萜类、4个糖苷、其他类(1个香豆素类、1个生物碱类、2个酯类、1个多酚类、2个核苷类),其中通过与对照品对比鉴定了8个化合物,分别为绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、马钱苷、马钱苷酸和樟芽菜苷。结论该研究首次采用UPLC-Q-TOF-MS/MS技术对彝族药双参的成分进行了研究,为其质量控制、药效物质基础及体内代谢物分析提供可靠的依据。OBJECTIVE To rapidly identify the chemical constituents of Triplostegia glandulifera Wall by ultra-performance liquid chromatography-electrospray/quadrupole time-of-flight tandem mass spectrometry(UPLC-ESI-Q-TOF-MS/MS).METHODS The separation was performed on Waters Acquity UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)column with gradient elution of 0.1%formic acid(A)-acetonitrile(B)at a flow rate of 0.4 mL·min^(-1).MS data were collected in positive and negative ion modes,the scanning range was m/z100-2000,and electrospray ionization(ESI)was employed.The chemical constituents of Triplostegia glandulifera Wall were identified by comparing the retention time and the mass data with those of the reference substances,as well as the accurate mass,MS/MS fragment ions,mass spectrometry databases(ChemicalBook,ChemSpider,Pubmed andothers)and related literature.RESULTS Fifty-nine compounds from Triplostegia glandulifera Wall were identified,including 11 iridoids,23 organic acids,14 triterpenoids,4 glycosides and others(one coumarin,one alkaloid,two esters,one phenolic acid and two nucleosides).Among them,eight compounds were identified by comparison with the reference substances,including chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C,loganin,ferulic acid and sweroside.CONCLUSION This research is the first one to study the chemical constituents of Yi nationality medicine Triplostegia glandulifera Wall,which provides a reference for the quality control,material basis and in vivo metabolic analysis.

关 键 词：彝族药 双参 超高效液相色谱-电喷雾/四极杆飞行时间串联质谱法 皂苷 环烯醚萜 有机酸 裂解规律 

分 类 号：R917[医药卫生—药物分析学]