钩藤多指标含量测定及化学计量学分析  

Muti-components Determination and Chemometric Analysis of Uncariae Ramulus Cum Uncis

作　　者：夏曼玉 张留记[1,2] 屠万倩[2] 李向阳[3] 王建霞 吴冰帆 XIA Man-yu;ZHANG Liu-ji;TU Wan-qian;LI Xiang-yang;WANG Jian-xia;WU Bing-fan(Henan University of Chinese Medicine,Zhengzhou 450008,China;Henan Engineering Center of Genuine Medicinal Materials Comprehensive Development,Henan Academy of Chinese Medicine,Zhengzhou 450004,China;Henan Provimcial Institute for Food and Drug Control,Zhengzhou 450018,China;Henan Health Care College,Zhengzhou 450008,China)

机构地区：[1]河南中医药大学,郑州450008 [2]河南省中医药研究院,河南省道地药材综合开发工程技术研究中心,郑州450004 [3]河南省食品药品检验所,郑州450018 [4]河南卫生健康干部学院,郑州450008

出　　处：《中国药学杂志》2022年第19期1621-1627,共7页Chinese Pharmaceutical Journal

基　　金：河南省科技厅工程技术研究中心建设项目资助(2020338);河南省中医专项课题重大专项资助(2019ZYZD08)。

摘　　要：目的建立同时测定钩藤中绿原酸、异钩藤碱、钩藤碱、去氢毛钩藤碱、毛钩藤碱、喜果苷含量的方法,基于化学计量学分析,评价钩藤的质量。方法采用高效液相色谱(HPLC),KinetexC色谱柱(4.6 mm×100 mm,2.6μm),以乙腈(A)-0.1%磷酸水(含0.5 g·L^(-1)1-丁基-3-甲基咪唑氯盐)(B)为流动相,梯度洗脱,流速:0.8 mL·min^(-1);检测波长327 nm(绿原酸)、246 nm(异钩藤碱、钩藤碱、去氢毛钩藤碱、毛钩藤碱和喜果苷);柱温:35℃;进样量:10μL。结果绿原酸、异钩藤碱、钩藤碱、去氢毛钩藤碱、毛钩藤碱和喜果苷分离良好,平均回收率分别为99.74%、103.25%、98.35%、99.45%、104.16%、97.98%。11批钩藤上述成分的含量测定结果分别为0.1287~0.4320、0.0071~0.0221、0.0081~0.0237、0.0098~0.0471、0.0057~0.0228、0.0089~0.0349 mg·g^(-1)。化学计量学分析表明不同产地的钩藤质量存在一定差异。结论建立的钩藤多指标含量测定方法简单、准确,可为钩藤药材质量综合评价提供新的参考方法。OBJECTIVE To establish a method for simultaneous determination of chlorogenic acid,isorhychophylline,rhynchophylline,hirsuteine,hirsutine,and vincosamide in uncariae ramulus cum uncis,and to evaluate its quality based on chemometric analysis.METHODS HPLC was used.The analysis was performed on a Kinetex Ccolumn(4.6 mm×100 mm,2.6μm),with the mobile phase consisted of acetonitrile(A)-0.1%phosphoric acid water(containing 0.5 g·L^(-1)1-buty-3-methylimidazolium chloride)(B)in gradient elution mode.The flow rate was 0.8 mL·min^(-1),and the detection wavelength was set at 327 nm for chlorogenic acid)and 246 nm for isorhychophylline,rhynchophylline,hirsuteine,hirsutine and vincosamide.The column temperature was maintained at 35℃,and injection volume was 10μL.RESULTS Chlorogenic acid, isorhychophylline, rhynchophylline, hirsuteine,hirsutine,and vincosamide were separated well,with the average recovery rates of 99.74%,103.25%,98.35%,99.45%,104.16%and 97.98%,respectively.The contents of chlorogenic acid,isorhychophylline,rhynchophylline,hirsuteine,hirsutine,and vincosamide in 11 batches samples from different habitats were 0.1287-0.4320,0.0071-0.0221,0.0081-0.0237,0.0098-0.0471,0.0057-0.0228 and 0.0089-0.0349 mg·g^(-1),respectively.Chemometric analysis showed that there were certain quality differences among the samples from different areas.CONCLUSION The method is simple and accurate.It can provide a new reference method for the comprehensive quality evaluation of uncariae ramulus cum uncis.

关 键 词：钩藤 多指标成分 含量测定 高效液相色谱法 化学计量学 

分 类 号：R917[医药卫生—药物分析学] R282[医药卫生—药学]