miRNA-155-5P靶向TLR4基因对脓毒症急性肺损伤大鼠的干预作用  

Intervention effect of miRNA-155-5P targeted TLR4 gene on rats with sepsis acute lung injury

作　　者：刘彬[1] 马宏境[2] 张利华 曾妮[2] 景丽丽 LIU Bin;MA Hong-jing;ZHANG Li-hua;ZENG Ni;JING Li-li(Tianjin Fifth Central Hospital,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院急诊科,天津300450 [2]天津市第五中心医院呼吸科,天津300450

出　　处：《中华医院感染学杂志》2022年第17期2561-2565,共5页Chinese Journal of Nosocomiology

基　　金：天津市医院协会资助项目(2016009)。

摘　　要：目的 探究miRNA-155-5P靶向TLR4基因对脓毒症急性肺损伤大鼠的干预效果。方法 48只SPF级SD大鼠,分为空白组、模型组、过表达组、沉默组各12只,空白组不做任何处理,模型组、过表达组、沉默组构建脓毒症急性肺损伤大鼠模型,做miRNA-155-5P慢病毒滴定,分别进行干预。实时荧光定量聚合酶链式反应检测miRNA-155-5P基因表达量,酶联吸附法检测过氧化物酶(MPO),丙二醛(MDA)、B淋巴细胞瘤-2(Bcl-2)、高迁移率族蛋白1(HMGB1)水平,采用免疫印迹法(WB)检测IKKα/NF-κB信号通路蛋白表达量。结果 与过表达组比较,沉默组miR-155-5p表达量及MPO、MDA、Bcl-2、TLR4水平均降低(P<0.05)。与过表达组比较,沉默组HMGB1、JAK2、IKKα、NF-κB、STAT3水平均升高(P<0.05)。结论 沉默组脓毒症急性肺损伤大鼠miRNA-155-5P表达,能够靶向调控TLR4基因,通过对IKKα/NF-κB信号通路的作用,抑制氧化应激及炎症反应,发挥保护脓毒症急性肺损伤大鼠肺功能屏障的作用,对脓毒症急性肺损伤大鼠能够起到良好的干预作用。OBJECTIVE To explore the intervention effect of miRNA-155-5 P targeted TLR4 gene on rats with sepsis acute lung injury. METHODS Totally 48 SPF SD rats were divided into the blank group, the model group, the overexpression group and the silence group, with 12 rats in each group. The blank group was not given any treatment, the model group, the overexpression group and the silence group were treated with construction of models of rats with sepsis acute lung injury and were given miRNA-155-5 P lentivirus titration. The expression of miRNA-155-5 P gene was detected by means of real-time fluorescence quantitative polymerase-chain-reaction, the levels of peroxidase(MPO), malondialdehyde(MDA), B-lymphoma-2(Bcl-2) and high mobility group box 1(HMGB1) were detected by enzyme-linked immunosorbent assay, and the expression level of IKKα/NF-κB signaling pathway protein was detected using Western blot(WB) method. RESULTS The expression level of miR-155-5 p and the levels of MPO, MDA, Bcl-2 and TLR4 were significantly lower in the silence group than in the overexpression group(P<0.05). The levels of HMGB1, JAK2, IKKα, NF-κB and STAT3 of the silence group were significantly higher than those of the overexpression group(P<0.05). CONCLUSION Silencing the expression of miRNA-155-5 P in rats with acute lung injury in sepsis can target and regulate TLR4 gene, inhibit oxidative stress and inflammatory response through the effect of IKKα/NF-κB signaling pathway, and protect the lung function barrier and achieve the intervention effect on the rats with sepsis acute lung injury.

关 键 词：miRNA-155-5P 脓毒症急性肺损伤 IKKα/NF-κB通路 抑制氧化 肺功能 

分 类 号：R33[医药卫生—人体生理学]