辽宁省玉米穗腐病致病菌新知镰孢的分离鉴定与生物学特性研究  

Isolation,Identification and Biological Characteristics of Fusarium andiyazi Causing Maize Ear Rot in Liaoning

作　　者：黄诗涵 徐靖茹 高维达 王洪川 杨雪 肖淑芹[1] 薛春生[1] HUANG Shi-han;XU Jing-ru;GAO Wei-da;WANG Hong-chuan;YANG Xue;XIAO Shu-qin;XUE Chun-sheng(College of Plant Protection,Shenyang Agricultural University/Key Laboratory of Plant Immune in Liaoning Province,Shenyang 110161,China)

机构地区：[1]沈阳农业大学植物保护学院/辽宁省植物病理重点实验室,沈阳110161

出　　处：《玉米科学》2022年第5期129-133,共5页Journal of Maize Sciences

基　　金：国家重点研发计划项目(2018YFD0300307、2017YFD0201805)。

摘　　要：通过形态学和分子生物学方法鉴定菌株Fa11-9。结果表明,菌株Fa11-9产生的大型分生孢子镰刀形,3~5个分隔;小型分生孢子长椭圆形,单胞,延伸因子与新知镰孢聚为一类,确定菌株Fa11-9为新知镰孢(Fusarium andiyazi)。通过接种与再分离鉴定试验,完成柯赫式法则。利用十字交叉法和血球计数板计数法研究营养和环境条件对新知镰孢生长速度和产孢的影响,结果表明,菌丝生长温度范围为15℃~40℃,最适温度为25℃;光照条件对菌丝生长影响不显著;生长最适碳、氮源分别为麦芽糖、硝酸钠,促进分生孢子产生的最适碳、氮源分别为蔗糖、酵母。新知镰孢在辽宁省可引起玉米穗腐病。Fa11-9 strain was identified by morphological and molecular methods to demonstrate Fusarium andiyazi causing maize ear rot.The results showed that micro-conidia of Fa11-9 were sickle with 3-5 separates,mini-conidia oblong with a single cell.Sequences of elongation factor had 99.5%identification with that of F.andiyazi.Fa11-9 strain was identified as F.andiyazi.The effects on nutrient and environmental conditions to growth and conidiation of F.andiyazi were studied with cross and hemacytometer methods.The results showed that mycelia growth of F.andiyazi was 15℃-40℃,and the optimum temperature was 25℃.There was no significant difference on mycelia growth under different light conditions.Maltose and sodium nitrate were the optimum carbon and nitrogen sources to promote mycelia growth,and sucrose and yeast were the optimum carbons and nitrogen sources to conidiation,respectively.F.andiyazi causing maize ear rot was reported firstly in Liaoning province.

关 键 词：玉米 玉米穗腐病 新知镰孢 生物学特性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]