珍珠李枝枯病病原鉴定及致病性测定  

Identification and pathogenicity test of the pathogens of branch blight on pearl plum

作　　者：黄荣 唐利华[1] 郭堂勋[1] 李其利[1] 孙文秀[2] 黄穗萍[1] 陈小林[1] 莫贱友[1] HUANG Rong;TANG Lihua;GUO Tangxun;LI Qili;SUN Wenxiu;HUANG Suiping;CHEN Xiaolin;MO Jianyou(Institute of plant protection,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007,China;Yangtze University,Jingzhou 434025,China)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,南宁530007 [2]长江大学生命科学学院,荆州434025

出　　处：《植物病理学报》2022年第5期863-866,共4页Acta Phytopathologica Sinica

基　　金：广西重点研发计划(桂科AB20159041);广西作物病虫害生物学重点实验室自主研究课题(20-065-30-ST-04)。

摘　　要：李(Prunus salicina Lindl.)是蔷薇科、李属植物,种类繁多,分布范围广,性状优良,是我国重要的果树之一[1]。‘珍珠李’是天峨县从当地野生李中选育出来的晚熟优良李品种,是产区农民脱贫致富的重要产业之一,‘龙滩珍珠李’享有“李族皇后”的美誉[2]。然而,随着‘珍珠李’种植面积的不断扩大和种植年限的增加,叶枯病、枝枯病等的发生日益增多,为害日趋严重[3]。In order to confirm the pathogen of branch blight on pearl plum and its virulence,14 samples were collected from Guangxi and Guizhou Province,and 21 isolates were obtained and were identified as Botryosphaeriaceae.The results of colony morphology,conidial observation and molecular identification of internal transcribed spacer region(ITS1-5.8 S-ITS2)and the translation elongation factor 1-α(EFl-α)showed that the pathogens of branch blight on pearl plum were Botryosphaeria dothidea(66.67%),Neofusicoccum parvum(19.05%),N.algeriense(9.52%),and Lasiodiplodia pseudotheobromae(4.76%).The pathogenicity of different species of Botryosphaeriaceae was tested by mycelial dishes inoculation,and it was found that typical isolates of the four species were pathogenic,among which L.pseudotheobromae was the most virulent one and B.dothidea was the weakest one.This is the first report that L.pseudotheobromae causes branch blight on pearl plum.

关 键 词：枝枯病 致病性测定 病原鉴定 叶枯病 珍珠李 种植年限 李品种 李属植物 

分 类 号：S432.44[农业科学—植物病理学]