苹果茎痘病毒RdRp基因分子变异分析  

Molecular diversity analysis of RdRp gene of apple stem pitting virus

作　　者：马小方 洪霓[2] 蒋迎春[1] 吴黎明[1] 宋放[1] 何利刚 王策[1] 于婷婷 王志静 王国平[2] MA Xiaofang;HONG Ni;JIANG Yingchun;WU Liming;SONG Fang;HE Ligang;WANG Ce;YU Tingting;WANG Zhijing;WANG Guoping(Research Institute of Fruit and Tea,Hubei Academy of Agricultural Science,Wuhan 430064,China;Key Laboratory of Plant Pathology of Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所,武汉430064 [2]华中农业大学植物科学技术学院,湖北省作物病害检测与安全控制重点实验室,武汉430070

出　　处：《植物病理学报》2022年第5期867-870,共4页Acta Phytopathologica Sinica

基　　金：国家重点研发计划(2020YFD1000100);湖北省农业科技创新中心创新团队(2019-620-000001-023);湖北省特色水果创新项目(2018TSG000)。

摘　　要：苹果茎痘病毒(apple stem pitting virus,ASPV)是凹陷病毒属β线性病毒科(Foveavirus,Betaflexiviridae)的典型代表。ASPV基因组是正单链RNA(+ssRNA),全长约9300个核苷酸,包含5个开放式阅读框(Open reading frames,ORFs),5′端非翻译区(Untranslated region,UTR)和3′UTR。ORF 1编码依赖RNA的RNA聚合酶(RNA dependent RNA polymerase,RdRp)。To elaborate the molecular diversity of pear-infecting ASPV in China,partial RNA dependent RNA polymerase(RdRp)fragments(P1 and P2)of four ASPV isolates were analyzed.We sequenced 17 P1 sequences and 14 P2 sequences of RdRp from 4 different isolates.Phylogenetic trees analysis based on these sequenced clones from our study and related sequences retrieved from the GenBank suggested that:1)ASPV grouping in phylogenetic trees were related to their hosts;2)ASPV isolates could be divided into five evolu-tionary divergent subgroups(A-E)based on partial RdRp fragments(P1 and P2),wherein one new subgroups(D)was identified in our study based on partial RdRp fragments P1.Recombination events were detected in RdRp sequences in our study.Results from our study provide important information for studying molecular characteristics of ASPV.

关 键 词：苹果茎痘病毒 病毒属 病毒科 

分 类 号：S432.41[农业科学—植物病理学]