盐度对大鳞副泥鳅抗氧化酶及组织结构的影响  

Effects of Salinity on Antioxidant Enzymes and Histological Structure of Paramisgurnus dabryanus

作　　者：李雪 赵建[1] 王玮欣 朱洪赓 陈建华 朱明 王海华[3] LI Xue;ZHAO Jian;WANG Wei-xin;ZHU Hong-gen;CHEN Jian-hua;ZHU Min;WANG Hai-hua(Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,P.R.China;Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,P.R.China;Fisheries Research Institute of Jiangxi Province/Scientific Observing and Experimental Station of Fishery Resources Environment in Poyang Lake,Ministry of Agriculture,Nanchang 330039,P.R.China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业农村部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点实验室,江苏连云港222005 [3]江西省水产科学研究所,农业部湖泊渔业资源环境科学观测实验站,江西南昌330039

出　　处：《水生态学杂志》2022年第6期139-145,共7页Journal of Hydroecology

基　　金：江苏省重点研发计划(现代农业)(BE2018387);江西省现代农业产业技术体系建设专项资金(JXARS-10);农业农村部热带亚热带水产资源利用与养殖重点实验室开放课题(2019PR03)。

摘　　要：为研究盐度对大鳞副泥鳅(Paramisgurnus dabryanus)Na^(+)-K^(+)-ATP酶(NKA)、抗氧化酶活性及组织结构的影响,试验设置4、8、12共3个盐度组和1个淡水对照组,以全长(17.60±0.69) cm、体重(35.51±5.30) g的大鳞副泥鳅为研究对象进行14 d胁迫试验。结果显示,盐度升高使大鳞副泥鳅的鳃Na^(+)-K^(+)-ATP酶活力上升;7 d时盐度8和12组Na^(+)-K^(+)-ATP酶活性均达到峰值,且显著高于淡水对照组(P<0.05)。肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均表现为先上升、后下降的趋势,且分别于胁迫12 h和2 d时达到最大值;3个盐度组的谷胱甘肽过氧化物酶(GSH-PX)活性在6 h和12 h均有所升高,且盐度12组显著高于淡水对照组(P<0.05)。盐度4、8和12组肝脏丙二醛(MDA)含量分别在1 d和7 d时达到最大值,且显著高于淡水对照组(P<0.05)。组织切片显示,盐度12组的鳃小片间距变大,泌氯细胞数量增多,上皮层被破坏甚至出现溶解现象;肝细胞空泡化严重,血窦扩张范围增大,并出现细胞轮廓模糊,细胞核偏移、溶解。研究表明,大鳞副泥鳅对水体盐度变化具有一定的调节适应能力,但盐度达到12时会对其鳃和肝组织结构造成一定损伤。Paramisgurnus dabryanus, belonging to Paramisgurnus, Cobitidae, is a common freshwater fish species, commercially valuable and highly nutritious. Recent research has found that P. dabryanus shows a degree of salt tolerance. This study explored the effect of salinity on the activities of Na^(+)-K^(+)-ATPase,antioxidant enzymes, and the histological structure of P. dabryanus, aiming to provide new ideas for the low-salt aquaculture of P. dabryanus in saline alkali waters. P. dabryanus of average total length(17.60±0.69) cm and body mass(35.51±5.30) g were selected for the test. Three salinity groups(4, 8, 12) and a control group were set, and each treatment was run in triplicate, with 20 test fish for each trial. The test lasted 14 d under the following conditions: water temperature 20-22℃, pH 7.60-7.90, dissolved oxygen5.2-6.8 mg/L and ammonia nitrogen 0-0.35 mg/L. During the test, the water of each treatment was changed in half daily with the corresponding salinity. After 6 h, 12 h, 1 d, 2 d, 3 d, 7 d and 14 d of the stress test, six fish were sampled for determination of antioxidant enzymes and observation of histological structure. The mortality rates of the control and salinity 4 groups were 0%, while the mortality rates of the salinity 8 and 12 groups were(6.67±2.88)% and(23.33±2.88)%, respectively. The activity of Na^(+)-K^(+)-ATPase in gills increased with salinity, and the activities of Na^(+)-K^(+)-ATPase in the salinity 8 and 12groups peaked on day 7 and were significantly higher than those in the control and salinity 4 groups(P<0.05). The activities of SOD and CAT increased initially and then decreased, peaking at 12 h and 2 d,respectively. The activity of GSH-PX in the three salinity groups all increased at 6 h and 12 h, and the activity of GSH-PX in the salinity 12 group was significantly higher than that in the control group(P<0.05). The contents of MDA in the salinity 4 and 8 groups peaked on 1 d and the salinity 12 group on 7 d,all significantly higher than the control group(P<0.05). Histolog

关 键 词：大鳞副泥鳅 盐度 Na^(+)-K^(+)-ATP酶 抗氧化酶 组织结构 

分 类 号：Q554[生物学—生物化学]