异牛肝菌素对人胃癌BGC-823细胞增殖及周期影响  

Effect of Iso-suillin on the Proliferation and Cycle of Human Gastric Cancer BGC-823 Cells

作　　者：徐彦楠[1] 赵青 赵俊霞[2] 周娜静[2] 高品 周晨明[3] XU Yannan;ZHAO Qing;ZHAO Junxia(Department of Teaching and Experiment Center,Hebei Medical University,Hebei 050017,China)

机构地区：[1]河北医科大学教学实验中心,石家庄050017 [2]河北医科大学细胞生物教研室,石家庄050017 [3]河北医科大学大型科研仪器设备共享服务平台,石家庄050017 [4]河北医科大学第四医院眼科,石家庄050011

出　　处：《医学研究杂志》2022年第11期119-123,共5页Journal of Medical Research

基　　金：河北省医学科学研究计划项目(20210149)。

摘　　要：目的探讨异牛肝菌素(iso-suillin)对人胃癌BGC-823细胞增殖及细胞周期的影响。方法体外培养人胃癌BGC-823细胞至对数生长期,分别使用12.5、25.0、50.0μg/ml的异牛肝菌素干预72h,同时设置对照组。CCK-8法测定人细胞增殖抑制率,流式细胞术碘化丙啶(PI)染色法检测细胞周期分布情况,Western blot法检测p21、p53、cyclinD1、CDK4蛋白表达,Hoechst 33342荧光染色观察细胞凋亡情况。结果随时间延长,12.5μg/ml剂量组、25.0μg/ml剂量组和50.0μg/ml剂量组A值均减小,细胞增殖抑制率均升高(P均<0.05);与对照组比较,12.5μg/ml剂量组、25.0μg/ml剂量组和50.0μg/ml剂量组不同时刻的A值减小,细胞增殖抑制率升高,且呈现出剂量依赖效应(P<0.05)。与对照组比较,12.5μg/ml剂量组、25.0μg/ml剂量组和50.0μg/ml剂量组细胞G_(0)/G_(1)期细胞比例增加,S期细胞比例减少,并且呈现出剂量依赖效应(P<0.05)。与对照组比较,12.5μg/ml剂量组、25.0μg/ml剂量组和50.0μg/ml剂量组p21、p53蛋白相对表达量升高,cyclinD1、CDK4蛋白相对表达量降低,且呈现出剂量依赖效应(P<0.05)。与对照组比较,12.5μg/ml剂量组、25.0μg/ml剂量组、50.0μg/ml剂量组细胞凋亡率升高,且呈剂量依赖性(P<0.05)。结论异牛肝菌素可抑制人胃癌BGC-823细胞增殖,使细胞周期阻滞于G_(0)/G_(1)期,同时促进其凋亡,其作用机制可能与上调p21、p53蛋白表达,下调cyclinD1、CDK4蛋白表达有关。Objective To investigate the effect of iso-suillin on the proliferation and cell cycle of human gastric cancer BGC-823 cells.Methods Human gastric cancer BGC-823 cells were cultured in vitro to the logarithmic growth phase and intervened with 12.5,25.0 and 50.0μg/ml of isobactin for 72h,while a control group was set up.The proliferation inhibition rate of human cells was measured by CCK-8 method,the cell cycle distribution was detected by flow cytometry propidium iodide(PI)staining,the expression of p21,p53,cyclinD1,CDK4 protein was detected by Western blot,and apoptosis was observed by Hoechst 33342 fluorescence staining.Results The A values of 12.5,25.0 and 50.0μg/ml dose groups decreased and the inhibition rate of cell proliferation increased with increasing time(P<0.05).Compared with the control group,the A values decreased and the cell proliferation inhibition rate increased at different moments in the 12.5,25.0 and 50.0μg/ml dose groups,and showed a dose-dependent effect(P<0.05).Compared with the control group,the proportion of cells in G_(0)/G_(1) phase increased and the proportion of cells in S phase decreased in the 12.5μg/ml dose group,25.0μg/ml dose group and 50.0μg/ml dose group,and showed a dose-dependent effect(P<0.05).Compared with the control group,the relative expression of p21 and p53 proteins was increased and the relative expression of cyclinD1 and CDK4 proteins was decreased in the 12.5μg/ml dose group,25.0μg/ml dose group and 50.0μg/ml dose group,and showed a dose-dependent effect(P<0.05).Compared with the control group,the apoptosis rate was increased in the 12.5μg/ml dose group,25.0μg/ml dose group and 50.0μg/ml dose group in a dose-dependent manner(P<0.05).Conclusion Isobiotin inhibites the proliferation of human gastric cancer BGC-823 cells and blockes the cell cycle in G_(0)/G_(1) phase,while promotes their apoptosis,and its mechanism of action may be related to the upregulation of p21 and p53 protein expression and downregulation of cyclinD1 and CDK4 protein expression.

关 键 词：异牛肝菌素 人胃癌BGC-823细胞 细胞增殖 细胞周期 细胞周期蛋白 CYCLIND1 

分 类 号：R28[医药卫生—中药学]