Blimp-1、SLAMF6表达异常与再生障碍性贫血患者免疫功能及发病的关系研究  

Relationship between abnormal expression of Blimp-1 and SLAMF6 and immune function and pathogenesis of aplastic anemia patients

作　　者：贺扬欣 陈颖[1] 王怀宇[1] 贺鹏程[1] 王晓宁[1] He Yangxin;Chen Ying;Wang Huaiyu;He Pengcheng;Wang Xiaoning(Department of Hematology,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院血液内科,西安710061

出　　处：《中华临床医师杂志（电子版）》2022年第4期312-318,共7页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金委员会资助项目(81600179)。

摘　　要：目的探讨再生障碍性贫血(AA)患者B淋巴细胞诱导成熟蛋白(Blimp-1)、重组人SLAM家族成员6(SLAM6)表达及意义。方法选取2017年1月至2019年6月在西安交通大学第一附属医院治疗的AA患者89例(AA组),其中非重型AA患者51例,重型AA患者38例,同时选取健康体检者50例作为对照组,采用RT-PCR检测Blimp-1 mRNA表达,流式细胞仪检查SLAMF6表达、CD3+、CD4+、CD8+、调节性T细胞(Treg)。结果AA组Blimp-1 mRNA相对表达量、SLAMF6表达、CD4+和Treg细胞分别为(0.65±0.20)、(55.10±11.82)%、(23.39±3.11)%和(3.03±0.90)%,明显低于对照组(P<0.05),而CD8+为(40.02±5.59)%,明显高于对照组(P<0.05);AA组重型患者Blimp-1 mRNA相对表达量、SLAMF6表达和Treg细胞分别为(0.45±0.12)、(47.02±11.04)%和(2.19±0.70)%,明显低于非重型患者(P<0.05),而CD8+为(47.54±7.21)%,明显高于非重型患者(P<0.05);Blimp-1 mRNA相对表达量与Treg呈正相关(r=0.589,P<0.05),SLAMF6表达与CD8+呈负相关(r=-0.320,P<0.05);AA组治疗后Blimp-1 mRNA相对表达量、SLAMF6表达、CD4+和Treg细胞分别为(0.82±0.16)、(62.01±10.12)、(32.02±5.09)%和(6.62±1.12)%,明显高于治疗前(P<0.05),而CD8+为(31.15±6.68)%,明显低于治疗前(P<0.05)。结论AA患者Blimp-1和SLAMF6表达下调,与病情严重程度有关,其中Blimp-1表达与Treg呈正相关,SLAMF6表达与CD8+呈负相关。Objective To explore the significance of expression of B-lymphocyte-induced mature protein(Blimp-1)and recombinant human SLAM family member 6(SLAMF6)in patients with aplastic anemia(AA).Methods From January 2017 to June 2019,89 AA patients(AA group)were selected at the First Affiliated Hospital of Xi'an Jiaotong University,including 51 non-severe AA patients and 38 severe AA patients.Fifty healthy people were selected as a control group.The expression of Blimp-1 mRNA was detected by RT-PCR,while the expression of SLAMF6 protein,CD3+T cells,CD4+T cells,CD8+T cells,and regulatory T(Treg)cells was detected by flow cytometry.Results The relative expression of Blimp-1 mRNA and the expression of SLAMF6 protein,CD4+T cells,and Treg cells in the AA group were(0.65±0.20),(55.10±11.82)%,(23.39±3.11)%,and(3.03±0.90)%,respectively,which were significantly lower than those of the control group(P<0.05),while the expression of CD8+T cells was(40.02±5.59)%,which was significantly higher than that of the control group(P<0.05).The relative expression of Blimp-1 mRNA and the expression of SLAMF6 protein and Treg cells in severe AA patients were(0.45±0.12),(47.02±11.04)%,and(2.19±0.70)%,respectively,which were significantly lower than those in non-severe patients(P<0.05),while the expression of CD8+T cells was(47.54±7.21)%,which was significantly higher than that of non-severe patients(P<0.05).The relative expression of Blimp-1 mRNA was positively correlated with the expression of Treg cells(r=0.589,P<0.05),and the expression of SLAMF6 protein was negatively correlated with the expression of CD8+T cells(r=-0.320,P<0.05).After treatment,the relative expression of Blimp-1 mRNA and the expression of SLAMF6 protein,CD4+T cells,and Treg cells in the AA group were(0.82±0.16),(62.01±10.12),(32.02±5.09)%,and(6.62±1.12)%,respectively,which were significantly higher than those before treatment(P<0.05),while the expression of CD8+T cells was(31.15±6.68)%,which was significantly lower than that before treatment(P<0.05).Conclusion

关 键 词：再生障碍性贫血 B淋巴细胞诱导成熟蛋白 重组人SLAM家族成员6 免疫功能 

分 类 号：R556[医药卫生—血液循环系统疾病]