中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用及氧化应激的影响  

Protective Effect of Bushen Jiedu Decoction Against Radiation-induced Placenta Injury and Oxidative Stress in Pregnant Mice

作　　者：高庆 李延晖 谭德慧 杨云霜 GAO Qing;LI Yanhui;TAN Dehui;YANG Yunshuang(Department of Traditional Chinese Medicine,PLA Rocket Force Characteristic Medical Center,Beijing 100088,China;Department of Traditional Chinese Medicine,Beijing Fulong Hospital,Beijing 100010,China)

机构地区：[1]中国人民解放军火箭军特色医学中心中医科,北京100088 [2]北京市隆福医院中医科,北京100010

出　　处：《世界中医药》2022年第22期3154-3157,3161,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(8170151209)。

摘　　要：目的:研究中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用及氧化应激的影响。方法:选取50只雌性以及25只雄性小鼠,待雌性大鼠受孕成功后,将其按照随机数字表法分为空白对照组,低剂量辐射组,中剂量辐射组,低剂量辐射+补肾解毒方组,中剂量辐射+补肾解毒方组,每组10只。低剂量辐射+补肾解毒方组和中剂量辐射+补肾解毒方组大鼠予以补肾解毒方灌胃干预,给药剂量为13.5 g/(kg·d)。空白对照组,低剂量辐射组,中剂量辐射组则予以等体积的生理盐水灌胃。各组连续灌胃10 d后,除空白对照组外,其余4组大鼠均予以γ射线照射,其中低剂量辐射与低剂量辐射+补肾解毒方组γ射线照射剂量为2 Gy,中剂量辐射组与中剂量辐射+补肾解毒方组γ射线照射剂量为4 Gy。比较各组孕鼠妊娠结局,仔鼠一般情况以及胎盘指数,孕鼠胎盘氧化应激指标水平。结果:低剂量辐射组与中剂量辐射组孕鼠的活胎率明显低于空白对照组,且死胎率高于空白对照组,而低剂量辐射+补肾解毒方组、中剂量辐射+补肾解毒方组孕鼠的活胎率明显高于低剂量辐射组与中剂量辐射组,且死胎率低于死胎率低剂量辐射组与中剂量辐射组(均P<0.05)。低剂量辐射组与中剂量辐射组仔鼠体质量、身长、尾长以及胎盘指数均明显低于空白对照组,而低剂量辐射+补肾解毒方组,中剂量辐射+补肾解毒方组仔鼠体质量、身长、尾长以及胎盘指数均明显高于低剂量辐射组与中剂量辐射组(均P<0.05)。低剂量辐射组与中剂量辐射组孕鼠超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平均低于空白对照组,而丙二醛(MDA)水平高于空白对照组;低剂量辐射+补肾解毒方与中剂量辐射+补肾解毒方组孕鼠SOD、GSH-Px水平均高于空白对照组,而MDA水平低于空白对照组(均P<0.05)。结论:中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用�Objective:To study the protective effect of Bushen Jiedu Decoction against placenta injury and oxidative stress in pregnant mice after radiation.Methods:Fifty female mice and 25 male mice were selected.After successful conception,female mice were divided into a blank control group,a low-dose radiation group,a medium-dose radiation group,a low-dose radiation+Bushen Jiedu Decoction group,and a medium-dose radiation+Bushen Jiedu Decoction group according to the random number table,with 10 mice in each group.Mice in the low-and medium-dose radiation+Bushen Jiedu Decoction groups received Bushen Jiedu Decoction at 13.5 g/(kg·d)for intervention,while those in the blank control group,and low-and medium-dose radiation groups were given the equal volume of normal saline by gavage.After 10 days of intervention by gavage,mice,except for those in the blank control group,underwentγray radiation.Specifically,the radiation dose in the low-dose radiation group and the low-dose radiation+Bushen Jiedu Decoction group was 2 Gy,and that in the medium-dose radiation group and the medium-dose radiation+Bushen Jiedu Decoction group was 4 Gy.The pregnancy outcome,general condition of neonatal mice,placental index,and oxidative stress indicators of pregnant mice of the two groups were compared.Results:In the low-and medium-dose radiation groups,the live birth rate was lower and the stillbirth rate was higher than those in the blank control group(P<0.05).In the low-and medium-dose radiation+Bushen Jiedu Decoction groups,the live birth rate was higher and the stillbirth rate was lower than those in the low-and medium-dose radiation groups(P<0.05).Compared with the blank control group,the low-and medium-dose radiation groups showed decreased body mass,body length,and tail length of neonatal mice,and placental index(P<0.05).Compared with the low-and medium-dose radiation groups,the low-and medium-dose radiation+Bushen Jiedu Decoction groups showed increased body mass,body length,and tail length of neonatal mice,and placental index(P<0.05).

关 键 词：辐射防护 补肾解毒方 妊娠结局 氧化应激 

分 类 号：R285.5[医药卫生—中药学]