HPLC法同时测定徐长卿及老君须提取物中咖啡酸、橙皮苷、白薇苷A和胡萝卜苷含量  

Simultaneous Determination of Caffeic Acid,Hesperidin,Cynatratoside A and Daucosterol in the Extracts of Cynanchum Paniculatum and Cynanchum Inamoenum by HPLC

作　　者：王建 侯金良 李正国 Wang Jian;Hou Jinliang;Li Zhengguo(Jining Center for Food and Drug Control,Shandong Jining 272025,China)

机构地区：[1]济宁市食品药品检验检测研究院,山东济宁272025

出　　处：《中国药师》2022年第10期1829-1833,共5页China Pharmacist

摘　　要：目的:建立HPLC法同时测定徐长卿及老君须提取物中咖啡酸、橙皮苷、白薇苷A和胡萝卜苷,并比较两种提取物中各成分的含量差异。方法:采用Agilent Zorbax C_(18)色谱柱(250 mm×4.6 mm,5μm),以0.15%磷酸水(A)-乙腈(B)为流动相进行梯度洗脱,流速1 ml·min^(-1),柱温40℃,检测波长为260 nm和210 nm。结果:咖啡酸、橙皮苷、白薇苷A和胡萝卜苷分别在2.1700~86.8000 mg·L^(-1)、5.4125~216.5000 mg·L^(-1)、3.5950~143.8000 mg·L^(-1)和4.0900~163.6000 mg·L^(-1)范围内线性关系良好。通过研究发现,徐长卿提取物中咖啡酸、橙皮苷、白薇苷A和胡萝卜苷的含量分别为2.0440,5.9540,3.9050,4.3100 mg·g^(-1),而老君须提取物中仅可检测到白薇苷A和胡萝卜苷,含量分别为4.953,7.672 mg·g^(-1)。结论:该方法简便、稳定,重复性好,可用于徐长卿及老君须提取物的质量评价。Objective:To establish an HPLC method for the simultaneous determination of caffeic acid,hesperidin,cynatratoside A and daucosterol in the extracts of Cynanchum paniculatum and Cynanchum inamoenum,and to compare the content differences between the two extracts.Methods:The analysis was carried out on an Agilent Zorbax C_(18) column(250 mm×4.6 mm,5μm)with 0.15%phosphoric acid(A)-acetonitrile(B)in a gradient elution mode as the mobile phase at a flow rate of 1 ml·min^(-1).The column temperature was 40℃and the detection wavelengths were 260 nm and 210 nm.Results:Good linear relationship was found within the range of(2.1700-86.8000)mg·L^(-1) for caffeic acid,(5.4125-216.5000)mg·L^(-1) for hesperidin,(3.5950-143.8000)mg·L^(-1) for cynatratoside A and(4.0900-163.6000)mg·L^(-1) for daucosterol.The study found that the contents of caffeic acid,hesperidin,cynatratoside A and daucosterol in the extracts of Cynanchum paniculatum were 2.0440 mg·g^(-1),5.9540 mg·g^(-1),3.9050 mg·g^(-1)and 4.3100 mg·g^(-1),respectively,while only cynatratoside A and daucosterol could be detected in the extracts of Cynanchum inamoenum,and their contents were 4.953 mg·g^(-1) and 7.672 mg·g^(-1),respectively.Conclusion:The method is simple,stable and reproducible,which can be used for the quality evaluation of the extracts of Cynanchum paniculatum and Cynanchum inamoenum.

关 键 词：徐长卿 老君须 咖啡酸 橙皮苷 白薇苷A 胡萝卜苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]