云南红河州番茄褐色皱纹果病毒检测  

Determination of Tomato Brown Rugose Fruit Virus in Honghe Prefecture of Yunnan

作　　者：吴阔[1] 陈永对[1] 王田田 吴少政 朱若萌 张仲凯[1] WU Kuo;CHEN Yongdui;WANG Tiantian;WU Shaozheng;ZHU Ruomeng;ZHANG Zhongkai(Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Agricultural Biotechnology/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture and Rural Affairs,Kunming,Yunnan 650205,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,云南昆明650205

出　　处：《贵州农业科学》2022年第12期71-75,共5页Guizhou Agricultural Sciences

基　　金：云南省省级“放管服”重大项目“云南主要天然香料植物资源的研究及产业化”(2019-1-N-25318000002120);云南省科技厅创新引导与科技型企业培育计划项目“云南省元谋县冬早春蔬菜产业科技特派团”(202104BI090023)。

摘　　要：【目的】建立云南红河州番茄褐色果病的检测技术,为控制其传播和治疗提供支撑。【方法】采用电子显微镜负染色观察、ELISA检测以及外壳蛋白基因序列RT-PCR检测及序列分析,明确云南红河州番茄褐色皱果病的病原。【结果】电子显微镜观察到病毒粒子为长300 nm、直径18 nm的杆状病毒,ELISA检测发现与烟草花叶病毒属的烟草花叶病毒(TMV)和番茄花叶病毒(ToMV)有血清学反应;RT-PCR扩增获得的目的片段与番茄褐色皱纹果病毒的病毒基因相似性达99.81%。【结论】引起云南红河番茄褐色皱纹果病的病原是烟草花叶病毒属的番茄褐色皱果病毒。【Objective】The detection technology of tomato brown rugose fruit virus in Hoghe Prefecture of Yunnan is established,so as to provide the support for controlling and preventing the virus.【Method】The electron microscope negative staining observation,ELISA testing,and RT-PCR detection and sequence analysis of the coat protein gene sequences are conducted to identify the pathogen causing tomato brown rugose fruit virus in Honghe Prefecture of Yunnan.【Result】Baculovirus with a length of 300 nm and diameter of 18 nm is observed by the electron microscope.ELISA testing shows the diseased fruit has a serological reaction with tobacco mosaic virus(TMV)and tomato mosaic virus(ToMV)of tobamovirus.The target fragment amplified by RT-PCR has a high similarity(99.81%)of virus gene with tomato brown rugose fruit virus.【Conclusion】The pathogen which is caused the tomato brown rugose fruit disease in Honghe Prefecture of Yunnan is the tomato brown rugose fruit virus of tobamovirus.

关 键 词：番茄褐色皱纹果病毒 烟草花叶病毒属 快速检测 细胞学 

分 类 号：S436.412.11[农业科学—农业昆虫与害虫防治]